Study On Regulation Of Immunocytes In Avian Inclusion Body Hepatitis

This study revealed molecular immunity mechanism of Avian Inclusion Body Hepatitis(IBH)by detecting the growth and decay or apoptosis regulation of macrophages and T,B lymphocytes in blood and immunity organ or liver,and also detecting the regulation of cells that express the adhesion molecules CD18. Two hundreds of one-day-old SPF chickens were inoculated FAV-8 mouth injection at a dose of 0.3ml per chicken,consisting of 1:50(w/v)tissuer virus suspension.And 10 of experimental group and 10 of control group were killed at 1,3,5,7,9,12,15,20,25 and 30 days postinfection respectively.All the lymphocyte suspension of peripheral blood,thymus,Fabricius bursa and spleen,were collected.And detected the growth and decay or apoptosis regulation of macrophage and T,B lymphocytes in blood and in variety immunity organ,and also detected the regulation of its adhesion molecules CD18 with flow cytometry. At the same time, liver and every immunity organ were fixed in 10% formalin for preparing waxy embedded sections and liver ice embedded sections,and to reveal the pathological changes and the regulation of lymphocytes or its subpopulations and macrophages in liver by the staining method of H.E. and immunohistochemistry . The research results that:(1) The IBH virus violates the liver of chicken primarily, showing the the view pathological changes of hemorrhagic and necrotic hepatitis mainly, and bleeds with the immunity organ.The disease develops again and again,and never completely recovered. (2)The Ia+ macrophage in blood was damaged mostly and to highest level at 5 day postinfection ,but the K1+ macrophages were in a positive situation all the time in blood or spleen. (3) The early apoptosis macrophages had arrived the highest level at 5 day postinfection, and the late apoptosis macrophages were significantly higher than control continuously; The early apoptosis lymphocytes were higher than control at 7 day postinfection, and then they were decreasing. (4)The Bu-1a+B lymphocytes in Fabricius bursa were cleared seriously,but it goes up significantly at the period of strong pathological changes in spleen and in blood . (5)Whether in thymus, spleen or in blood, the CD8+ T Lymphocytes were damaged mostly.Although the CD3+T and CD4+ T Lymphocytes had been harmed, the most of them were higher than control. TCRγδ+ T Lymphocytes in the blood and spleen had gone up significantly at the period of serious pathological changes, but they had greater drop in yield after the initial stage of virus attack and the strong pathological changes. (6)The adhesion molecule CD18 of macrophage was higher than control from 15 day postinfection to 20 day postinfection ,and then decreasing; the adhesion molecule CD18 of lymphocyte was higher than control at 20 day postinfection.(7) K1+ macrophages appeared at 3 day postinfection in liver, they arrived at the highest level at 9 day postinfection, and then they were decreasing; CD3+T lymphocytes also appeared at 3 day postinfection in liver, and they were increasing at 12 and 25 day postinfection respectively;CD4+T lymphocyte, the amount of them was increasing at 12 day postinfection;CD8+T lymphocytes, the amount of them was more than the CD4+T lymphocytes,;A little of TCRγδ+ T lymphocytes were appeared in liver.(8) This research examined the regulation of macrophages in blood or in spleen and its apoptosis in blood in the process of experimental IBH by flow cytometry for the first time; Examined the regulation of lymphocytes of experimental IBH in liver by the method of immunohistochemistry for the first time.