Flow Cytometry In Strepococcus Suis Adhesion Cell Research Application

Flow cytometry(FCM) is a function of the level of individual cells or other biological particles,or quantitative analysis of the test instrument separation.It high-speed analysis of tens of thousands of cells,and cells at the same time measuring the physical or chemical properties.Streptococcus suis is an important zoonotic pathogen.Streptococcus suis type 2(SS2) is the most common and most virulent serotype.SS2 can cause not only pig meningitis,arthritis,endocarditis,septicemia, pneumonia and sudden death,also died of infection and related personnel.In this study,gram-positive bacteria Streptococcus suis type 2(SS2) model for the study,the use of CFDA-SE cell proliferation and tracer tag Detection Kit SS2, will be green with CFDA-SE fluorescence SS2 with Hep-2 cells and RAW macrophages to use flow cytometry analysis of SS2 comparison of wild and mutant strains of the differences with a view to identifying with the adhesion of SS2 diabetes-related genes.At the same time,conventional plate count method SS2 adhesion Hep-2 cells and RAW macrophages,the results with flow cytometry results.The results showed that CFDA-SE had good effect on SS2,marking the time required for short,and the stability of fluorescent bacteria in vivo.The results of flow cytometry and plate count results were significantly correlated.However,flow cytometry method is simple,low-cost,accurate results,and good repeatability. Comparison of the wild-type,respectively,and the SC19 mutant SSP,2706,11-12, 196B of the cell adhesion capacity.SS2 Preliminary results show that wild-type SC19 on the Hep-2 cell adhesion ability,the ability of RAW cell adhesion in general;SSP mutant missing the capacity of the surface adhesion protein and the relationship was not obvious;196B by 2706 and the loss of the surface adhesion protein and the ability relatively close relationship.11-12 deletion mutant is restricted to limit the gene repair system of Clostridium perfringens strains and thickening of their ability to have a certain influence adhesion.