A Preliminary Study On Conservation Technology Of Edible Lily In Vitro

Edible lily is one of vegetative vegetables. The conservation of edible lily germplasm with the traditional method needs to replant every year. This method not only needs a lot of labor and money, but also is easily affected by diseases and insect pests, natural disasters. In order to settle those problems, the preservation in vitro of edible lily was explored in this paper. The establishment of propagation system and the effects of some key factors affecting the preservation quanlity of edible lily germplasm were involved in the preservation in vitro. The cryopreservation method of edible lily germplasm was also studied. The main results were as follows: The conservation of lily plantlets based on micropropagation of edible lily squama was tired on in 24 different MS medium under different temperatures (2,5,10,25). The results sho- wed: the plantlets that were conserved for 6 months at low temperature(2±1℃ and 5±1℃) all can survive.The SN7 that was appended with MNT was the best medium for the plant- lets and the SA5 that was appended with ABA was the best medium for the plantlets at 2±1℃ and 5±1℃.All plantlets conservered on the medium appended with ABA at 25±1℃and 10 ±1℃with 8h/d photoperiod 6 months almost died. The plantlets conserved on the medium appended with MNT must be step-culture. ABA and MNT was well growath depr- essor for lily preservation in vitro at 4 different temperatures. MNT was better than ABA. This is bec- ause the plantlets conserved in the medium appended with ABA were too depre- ssed and the plantlets in the medium appended with MNT are stuggy.The basic condition for the cryopreservation of lily shoot tips by vitrification was stu- died. The results indicated that the major factors determining whether the lily cryopreserved were successful or not were pre-teament and thawing methods of the shoot tips. The proced- ure and corresponding conditions obtained in the study were as follows: the shoot-tips were precultured on MS medium with 0.5M sucrose for 2 days and detached growing tip tissue with the size of 2～3mm was dehyrated with a vitrification solution (100%PVS2 Solu tion) for 20 minutes at 0℃. After new vitrification solution was replaced. Then plunge into liquid nitrogen. We got the highest survival rate (52.6%) after cryopreservation. The materials were plunged into liquid nitrogen. After 48h,The material was taken off from liquid nitrogen and thawed in 40℃ was cultivated on MS + 6-BA 0.5mg·L-1+NAA 0.1mg·L-1 +GA 0.3 mg·L-1+ sucrose 30g·L-1 +agar 7g·L-1The highest survival rate of tips after cryopreservation was 52.6%.