In Vitro Conservation Of Wild Lily

China is rich in wild lily (Lilium) germplasm resources. These lilies have some special characters and excellent genes, which served as essential sources for lily breeding. Generally, growing the bulbs in the field adopted as the main protection way to wild lily germplasm conservation. However, planting lily in the ground is vulnerable to suffer the natural threats and diseases and the genetic resources will lost at last. Therefore, new protection approaches aiming to lily (Lilium spp.) resources conservation need to be improved and explored.The dissertation was focused on the five species of wild lily in China, L. lancifolium, L. leichtlinii var. maximowiczii, L. pumilum, L. duchartrei, L. concolor var. pulchellum and researched the in vitro rapid propagation and short-term conservation by subculture, restrict-growth conservation, and cryopreservation of them. The results were below:(1) Developed the in vitro rapid propagation using lily scales, bulblets, leaves as the explants.The optimum medium for directly inducing the small lily bulbs from the explant were:MS+NAA1.0mg·L-1+TDZ0.2mg·L-1for L. lancifolium scales TCLs (thin cell layers), MS+NAA1.0mg·L-1+TDZ0.1mg·L-1+e-BA1.0mg·L-1for L. lancifolium bulblets, MS+NAA0.2mg·L-1+6-BA1.0mg·L-1for L. leichtlinii var. maximowiczii scales, MS+NAA2.0mg·L-1+TDZ0.2mg·L-1for L. pumilum scales, MS+NAA1.0mg·L-1+TDZ0.1mg·L-1for L. duchartrei leaves, MS+NAA2.0mg·L-1+TDZ0.2mg·L-1for L.concolor var. pulchellum scales TCLs. Subculture the bulbs on the medium of1/2MS+NAA0.5mg·L-1+AC2g·L-1for rooting.(2) Developed using the normal subculture methods for lilies in vitro conservation.At the normal condition(temperature is23±2℃, illumination intensity is40μmol·m-2·s-1and illumination time is14h·d-1), the five lily species can realize short-term preservation using subculture(3) Developed the restrict growth conservation for L. leichtlinii var. maximowiczii.At normal condition, the plantlet of L. leichtlinii var. maximowiczii grew slowly on the MS medium added with90g·L-1sucrose and another30g·L-1mannitol, and can be conserved at least6month without subsulture. After6months, the plantlet can recover growth on normal medium and the scales can induce shoots on induction medium. L. leichtlinii var. maximowiczii plantlet introducing from scales tissue culture can be preserve in MS medium with high concentration of sucrose and mannitol at least6months.(4) Developed the cryopreservation by vitrification for L. lancifoliumWhen the bulb grow on the medium for1～2cm, mini-bulb with one or two scales were put on the medium with0.3M sucrose for cold treatment one week. Shoot-tips were cut from the bulbs, and treated with loading solution for20min.Then,the shoot-tips were immense into the PVS2solution at0℃for100min before plunging into liquid nitrogen. Following by a rapid water bath, the shoot-tips were washed in unloading solution for lOmin twice. Shoot-tips can regrow on the hormone-free medium. Eight weeks cold treatment is necessary for the in vitro growth plantlets for transplanting into the earth. Cryopreservation by vitrification is an approach for L. lancifolium long-term preservation.The dissertation was focus on in vitro conservation of five species of wild lily. These lilies can be in vitro conserved, and the technologies that were use will be bringing more lilies conservation off body in the future.