| Glycyrrhiza uralensis Fisch., G. inflata Bat., 6. glabra Linn, and 6. pallidifiora Maxim, are Glycyrrhiza genus plants which belong to Fabaceae family. As the saying goes, "nine in ten prescriptions by Chinese herbalist doctors" the Glycyrrhiza, especially G. uralensis are known as "traditional Chinese medicine"; they also have the reputation of "the master-hand of anti-droughts, anti-sand and anti-winds". About the four Glycyrrhiza species in China, the author systemically summarized their studies in geographical distribution, medicament, livestock agriculture, food, cosmetic, industry, tobacco, ecological balance maintaining, resource, plant primary and secondary metabolites in particular. Through the review, the author has got the idea of the general situation of the studies on the four Glycyrrhiza species in China, which gave us a base to start the subject. We have been working on the subjects "studies on the deep exploitation and synthetical utilization of the full ingredients about the Glycyrrhiza species" which was supported by the science fund of Gansu province and "study on the application of good resource plants -five species of Glycyrrhiza" which was supported by the fund of the education committee of Gansu, The purpose of the studies was to provide a solid and scientific proof for the further exploitation and utilization of them.Chapter II: Studies on the essential constituents from the roots and leaves of 6. uralensis, G. Mats, 6. glabra and G. pallidifioraThe essential oil from the roots and leaves of 6. uralensis, G. inflata, G. glabra and G. pallidifiora was obtained by steam distillation. The chemical components were separated and identified by GC-MS and elucidated on the standard mass spectral data. The relative content of each component in percentage was calculated by area nomolization. 414 peaks were separated and 376 compounds were identified, the identification rate was 90.82%. It is the first time that this kind of studies was reported and it provided a solid and scientific proof for the further development of them.Chapter III: Extraction and determination of total triterpene and fiavonoids constituents isolated from the four Glycyrrhiza speciesThe methods of extraction and separation of total triterpene and fiavonoids constituents from the four kinds of Chinese Glycyrrhiza roots have been studied. The contents of total triterpene and fiavonoids have been detected through the weight method. Meanwhile, the concentration of the total fiavonoids in the extraction were also determined by the ultraviolet-visible spectrophotometry with three different methods. The results showed that the contents of total triterpene and fiavonoids varied with the population, the growing condition, the methods of extraction and separation, and even varied within the same population and group. Of the three methods, the concentration of total fiavonoids was higher by using the direct mensuration method, and the coloration method by AlCl3 and AI(NO3)3 was lower. Thesenquence of the total flavonoids contents in the crude extractions of the four species were G. uralensis, G. inflata, G. glabra and G. pallidifiora and that in the roots were G. glabra, G. uralensis, G. inflata and G. pallidifiora. The routes of the extraction and separation of total triterpene and flavonoids were quite suitable and the obtaining ratio was good.Chapter IV: Studies on the stability of pigment of G. uralensisIn this text, we mainly studied the extraction, separation and the stability on pigment of G. uralensis. The results showed that the stability of its pigment was good. It could endure heat and light, and exist in the medium of alkalescence. The research provided the basis for the development and utilization of pigment in G. uralensis. This pigment of G. uralensis will be a good natural pigment and be used in food and medicine.Chapter V: Electrochemical and UV-visible spectroscopic studies on the interactions between glycyrrhizic acid and the DNA from the leaves of Corydalis adunca, dl-tetrahydropalmatine and... |
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