| Soybean is one of the recalcitrant crops for in vitro culture. In the present study, the cotyledonary node regeneration system was tested, and three genotypes planted in southern China with high regeneration frequency were found. The major factors influencing the regeneration and Agrobacterium-mediated transformation were investigated.A reliable transformation system through the cotyledonary node regeneration was set up for the tested genotypes after the studies. A gene, gagal, regulating flower development were introduced into soybean through getting three PCR-positive transformed plants.Soybean somatic embroygenesis has been studied, and two genotypes were found with high embryogenetic potential.The main results can be summarized as below:1. In the cotyledonary node regeneration approach, regeneration frequencies are very different among genotypes. We have found ten genotypes with high regeneration frequency. M02 is the highest among them whose frequency is up to 80% while Changshusanzihuangdou takes the second position with frequency 73%.2. The susceptibility to Agrobacterium is very different among the tested genotypes. In the experiment, three genotypes with good susceptibility to Agrobacterium were found.3. Checking the effect of the construct of gaga1, we gained five transgenic tobacco plants, and their tobacco floral structure changed slightly.4. Among the fifteen survival soybean plants after transfering to soil, three plants gave positive PCR reaction. The genotype of one of the plants is Nannong 18-6 and that of the other two is Changshusanzihuangdou.5. Somatic embroygenesis was induced successfully from immature cotyledon. Two genotypes, Feixiheidou and Yunxiaowuzidou, were found with high embryogenesis potential. The procedure to induce secondary somatic embryos, propogate somatic embyos, and regenerate plantlets from somatic embryos has been completed.
|
PDF Full Text Request