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Effects Of Probiotics On Digestive Enzyme Activities, Intestinal Microflora And Performance Of Allogynogenetic Crucian Carp

Posted on:2005-02-20Degree:MasterType:Thesis
Country:ChinaCandidate:B LiuFull Text:PDF
GTID:2133360122993218Subject:Animal Nutrition and Feed Science
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To offer the direction of application of Bacillus licheniformis or Xylooligosaccharides and theory of the deep study in the aquaculture, effects of Bacillus licheniformis or Xylooligosaccharides on digestive enzyme activities, intestional microflora and performance of the fingerlings of Allogynogenetic Crucian Carp were studied.EXPERIMENT 1Effects of Bacillus licheniformis on intestine and hepatopancreas enzyme activities, performance of the fingerlings of Allogynogenetic Crucian CarpIn the experiment 375 allogynogenetic crucian carp finglings (average initial weight, 13.46 0.24) were allotted into five groups randomLy for 127 days. The first group was the control fed with basal diet, the others were the treated groups fed with basal diet addited 0.1 %o, 0.2%o, 0.3%o, 0.4%o Bacillus licheniformis respectively. The weight gain, activities of protease and amylase of the intestine, chyme and hepatopancreas, Hepatopancreas Somatic Indices were determined.The results showed Bacillus.licheniformis of 0.2%o, 0.3%o were the optimum growth for finglings of Allogygenetic Crucian Carp. The growth rates and feed conversion ratios of tested groups of 0.2%o, 0.3%oBacillus licheniformis were significant. (P<0.05), which were 13.14%, 17.38% higher and less 8.68%, 14.21% than those of the control respectively. Ingestion of 0.4%oBacillus licheniformis had no significant effects on the growth ratio (P>0.05), but the effect was significatnt on the feed conversion ratio(P<0.05), which decreased 10.00% than the control, and the others were not significant.In the subsequence experiment ,the growth ratio of the only 0.4%o Bacillus licheniformis was significant(P<0.05), which was 54.18% higher than that of the control.Compared with the control, ingestion of 0.2%o, 0.3%oBacillus licheniformis were significant (PO.05), the apparent digestibility of total dry matter(DM),crude protein and phosphorus increased 6.72%, 6.76% or 5.61%, 4.24% or 37.63%, 35.03%, respectively, butingestion of 0.1 %o, 0.4%oBacillus licheniformis had no significance( P>0.05 ). The activities of protease and amylase of chyme of treated groups of 0.2%, 0.3%o Bacillus licheniformis were significant(P<0.05), which were 120.16%, 138.01% and 41.08%, 40.71% higher than those of the control, respectively. The amylase activities of chyme of group of 0.4%o Bacillus licheniformis was 32.02% higher than that of the control(P<0.05). The protease activities in the intestine fed with dietary 0.2%o, 0.3%o, 0.4%oBacillus licheniformis were 95.80%, 142.76%, 157.52% higher than those of the control(P<0.05). The amylase activities of the intestine fed with dietary Bacillus licheniformis were not significant (P>0.05). The protease activities of the hepatopancreas fed with dietary Bacillus licheniformis were no-significance (P>0.05), but the amylase activities were significant (P<0.05), which were less 33.36%, 35.96%, 36.28%, 31.55% than those of the contrast.Compared with the control, Phytase activity of ingestion of Bacillus licheniformis were not significant(P>0.05), but they had the trend of increase, which improved 18.69%, 18.43%, 50.97%, 21.89%.0nly Ingestion of 0.4%oBacillus licheniformis, Hepatopancreas Somatic Indices(HIS) was significant(P<0.05), which was 20.91% less than the control, the others were not significant(P>0.05).EXPERIMENT2Effects of Bacillus licheniformis and Xylooligosaccharides on digestive enzyme activities, intestinal microflora and performance of the fingerlingsof Allogygenetic Crucian CarpThe experiment studied the effects of Bacillus licheniformis and Xylooligosaccharides (XOS) on performance, digestive enzyme activities of chyme and intestinal microflora of the fingerlings of Allogygenetic Crucian Carp. 60 Allogygenetic Crucian Carp (average weight 17.0g) were allotted into 3 groups randomly, namely I, II, III grouop for 60 days . The I ,II,IIIgroup were fed with basic diet, Q.2%oBacillus licheniformis, 0.1%oBacillus licheniformis and 0.1 %oXOS, respectively.The results showed ingestion of 0.2%o Bacillus licheniformis or the compound o...
Keywords/Search Tags:Bacillus licheniformis, Xylooligosaccharides (XOS), Digestive enzyme activities, Intestinal microflora, Performance, Allogynogenetic Crucian Carp
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