Effects Of Xylo-Oligosaccharides In Diets On The Weight Gain Ratio, Digestive Enzyme Activity And Immune Performance Of Allogynogenetic Crucian Carp

The study was conducted to examine effects and the mechanism of action of xylo-oligosaccharides(XOS) in diets in aquaculture. One-year-old AUogynogenetic Crucian Carp fingerling was selected as test animal in the study. The paper included three experiments:In the first experiment, 120 one-year-old AUogynogenetic Crucian Carp fingerlings (average initial weight ,17g) were randomly allotted into four treatments for 45 days. Treatment 1 was the control group fed with the basal diet, the others were the treated groups fed with diets containing 0.005%, 0.01%, 0.02% XOS respectively. The weight gain ratio and digestive enzyme activity in intestinal canal and in hepatopancreas were determined.The results showed 0.01% was the optimal adding dosis for one-year-old AUogynogenetic Crucian Carp fingerling under the indoor farm environment.Containing 0.01% XOS in the basal diet, weight gain ratio, intestinal amylase activity and proteinase activity in hepatopancreas of AUogynogenetic Crucian Carp fingerling increased significantly(p<0.05).Surpass this adding dosis, the weight gain ratio and digestive enzyme activity in intestinal canal and in hepatopancreas showed descending tendency .In the second experiment,90 one-year-old AUogynogenetic Crucian Carp fingerlings (average initial weight ,17g) were randomly allotted into three treatments for 45 days. Treatment 1 was control group fed with basal diet, group 2 and group 3 were the treated groups fed with diets congtaining 0.02% Bacillus licheniformis,combination of 0.01% Bacillus licheniformis and 0.01% XOS respectively. The weight gain ratio, digestive enzyme activity in intestinal canal and in hepatopancreas and the intestinal flora's populations were determined. The results showed, adding 0.02% Bacillus licheniformis ,the weight gain ratio,intestinal digestive enzyme activity of AUogynogenetic Crucian Carp fingerling increased by 75.94%, 24.43% (P<0.05) and 58.56% (P<0.05) respectively under the indoor farm environment,had no significant effect on digestive enzyme activity in hepatopancreas.The alteration of the intestinal flora's populations differed significantly from the control group ( P<0.05 ) .The populations of Bacillus licheniformis andLactobacillus increased by 8.86%, 24.37% respectively.The Colibacillus' population decreased by 9.23%.Adding the combination of 0.01% XOS and 0.01% Bacillus licheniformis, the weight gain ratio, the intestinal amylase activity and the proteinase activity in hepatopancreas increased by 80.72%, 55.92%, 82.02% respectively.The intestinal proteinase activity and the amylase activity in hepatopancreas increased by 16. 05%, 16.55% respectively (P>0.05).The Bacillus licheniformis' population decreased by 2.80% and the Lactobacillus' population increased by 4.52%.The Colibacillus' population decreased by 10%.In third experiment, 2100 one-year-old Allogynogenetic Crucian Carp fingerlings (average initial weight ,107g) were randomly allotted into seven treatments for 85 days. Treatment 1 was control group fed with basal diet, the others were the treated groups fed with diets containing 0.01% XOS, 0.02% XOS, 0.01% XOS+0.01% Bacillus licheniformis, 0.01% XOS+0.01% Bacillus licheniformis +0.03% multiplex oligsaccharides,0.005% XOS +0.005% Bacillus licheniformis +0.05% multiplex oligsaccharides, 0.005% XOS +0.005% Bacillus licheniformis +0.025% multiplex oligsaccharides +0.05% extrinsic compound enzyme preparation respectively.The weight gain ratio, intestinal digestive enzyme activity, digestive enzyme activity in hepatopancreas , immune performance and hepatopancreas indices were determined.The results showed , the weight gain ratio of Allogynogenetic Crucian Carp fingerling didn' t increase fed with diets containing XOS under the pond farm environment.The intestinal digestive enzyme activity,proteinase activity in hepatopancreas and activity of serum Lysozyme Allogynogenetic Crucian Carp fingerling increased significantly fed with diets containing 0.01% XOS. the amylase activity in hepatopancreas decreased and activities of serum SOD, serum ACP, seru...