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Microbial Bioremediation In Breed Aquatics Pond

Posted on:2004-12-12Degree:MasterType:Thesis
Country:ChinaCandidate:X J ZhangFull Text:PDF
GTID:2133360122993250Subject:Microbiology
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Now centralized and high-disenty breed aquatics have brought about severe pollution, which led to large loss in fishery. Some kinds of methods were used to get rid of the pollution. In the paper the microble bioremediation was tried to solve the problem in the breed aquatics pond.In the paper, The strain of A3, A32, B5. B23. B28 were identified as Bacillus, B. licheni formis, B. pumilus^ B. cereus. B. pscudomycoudes, B. weihenstephanenses respectively.By the single factor and orthorital experiment, the suitable culture medium of A3 was:1% starch; 0. 1% NH1NO4 ; 0.5% soja powder; 0.15%yeast cream; pH7. 0;the suitable culture medium of A32 was: 1% sucrose: 0. 1% NH4NO3 ; 0.5% soja powder; 0.05%yeast cream; pH7.O; the suitable culture medium of B5 was: 1.3% starch; 0.3% NaNO3 ; 0.5% so ja powder; 0.05% yeast cream; pH7. 0; the suitable culture medium of B23 was: 0.7% sucrose; 0.3% (NH4)2S4 ; 0.5% soja powder; 0. 15%yeast cream; pH7. 0; the suitable culture medium of B28 was: 1% sucrose; 0.1% NH4NO3: 0.3% soja powder; 0.05% yeast cream; pH7. 0.The experimental strains can remove th>: ammoniac nitrogen, the nitrite initial-ni trogeru the nitrate-ni trogen and the carbohydrate pollution not only in the aquaria but also in the breed aquaties pond.The innovation in the paper is that the remove of nitrogen was regarded as a standard for the microbiological bieremediation in the breed aquatics pond and that the microbes and the algae thai can be eat.en by the breed aquatics animals bioremediation in the breed aquatics pond was put forward, that is , the microbes decomposed the organic pollution into low molecular weight substance ,and the algae utilize the small molecular weight substance to grow , and the breed aquatics animals eat these algae. The relation of the strains and the algae SnedesomusyS2 microcystis aeruginosaW3 was researched .It was found that, the strains B23 can res: rain the growth of microcystis aerugmosaW3 in the BG reagent with sucrose or peptone and microcystis aeruginosaW3 was grown yellow and that the ferment liquid of B23 can restrain microcystis aeruginosaW3 to grow, but the strains B23 and B28 can not promote the growth of microcystis acruginosaW3 in the aquaria;and that the strains A3 A32 B5 can promote the SnedesoinusyS2 to grow in the HB-4 reagent with sucrose or peptone,but their influence was a little in the aquaria. It need more research in the strains and the algae bioremediation in the breed aquatics pond together.Their pathogenic effect on the animals such as crucian and the crops such as wheat and rice was not found.
Keywords/Search Tags:Microbe bioremediation, Strain identification, Breed aquatics pond, Decontaminate, Algae
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