| It is reported that the area of breed aquatics is increasing and the intensive culture is developing. However, the self-pollution of culture, which is arising from aquaculture wastewater, relict remain diet and feces deposit on the pond bottom, has become a severe ecological entironment problem.In the paper the carriers-microble bioremediation was tried to solve the problem in the aquatical pond. Based on the principle of immobilized microbial bioremediation, we selected the microbe compounds and carriers and tested the ability of carriers'adsorbtion of bacteria. Carrier-microbe compounds were applied in enclosure trial and shrimp ponds in order to testify the effect of bioremediation by carriers-microble.1. Under the condition of lab, we have made an experiment on the bioremediation of the organic polluted sediment by several ecology methods such as aerating oxygen, adding microbe compound and nutritious liquid. The results indicated that the rate of COD of bottom water in nutritious liquid (VB997) group was removed 70.00%, the value of G ranged from 12.00 kg/(kg·h)to 45.60 kg/(kg·h), and 1.80 to 2.20 cm oxidation stratum was shaped in the sediment surface. The group of aerating was full of oxygen, DO remain high than 7.00 mg/L, the rate of COD in the bottom water removing was 69.25%, the value of G ascend from 14.30 kg/(kg·h) to 34.20 kg/(kg·h). The rate of COD in microbe compound (LLMO) group was removed 61.11%, the value of G ranged increased 125%, and the oxidation stratum shaped was 1.20-1.90cm. The results show that different methods of bioremediation could degrade the organic compound in sediment and optimize the ecological environment.2. The abilities of adsorption of four candidates (DI, AC, CC and ZE) for the microbe compound were tested in the lab. The contrast experiment suggested that six-hour was the best time that carriers could adsorb the most bacteria: AC-carrier 183000 cfu/g, DI 177000 cfu/g, ZE 160000 cfu/g, CC 142000 cfu/g. Then we studied the abilities of carriers to degrade the organic compound of culture pond. The concentration of TOC in the ZE-M group and AC-M group were reduced 6.4% and 6.9%. The removal rates of COD, ZE-M group and AC-M group were 27% and 24.3% lower then that in the control. So ZE and AC were selected as microb compound's carriers.3. To testify the effect of bioremediation of polluted nature aquatics pond sediment, small enclosure trial was carried out in crucian farming pond in Mazhu-town Yuyao-city. According to the results, carrier-microbe compound could accelerate and the degrading of organic compound in water and sediment. The effect of AE-M was the best; the concentration of TOC was 14.25% lower then that in the control.4. Carrier-microbe compounds (AC-M and ZE-M) were applied in two shrimp ponds: one was marine culture pond in Xianxiang Yinzhou; another was freshwater culture pond in Mazhu Yuyao. The results indicated that carrier-microbe compound could degrade the feed and excreta of shrimp and decrease the organic compound in the ponds. The removal rates of COD in the ZE-M group and AC-M group were 32.5% and 29.87% lower then that in the control. The output in the experimental tank was as 1.5-2 times as that in the control. The famed shrimps were bigger and more active than those in control ponds. At the posterior time, the epidemic disease broke out in Xianxiang Yinzhou aera because of the weak fry and bad weather typhoon, but the disease erupted 12-day latter than in control ponds.The trial in lab and cultural pond suggested that adding carrier-microbe compound could degrade the organic pollution, improve the ecological environment, raise the output, and postpone the shrimp disease. Therefore, the full-scale use of this bioremediation technique could be a dependable technique to improve the cultural environment.
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