Studies On The Anthocyanins Of Impatiens Hawkeri Flowers

Impatiens hawkeri, as one of the popular flowers in the world, not only has high horticultural value, but also contains rich anthocyanins, which can be used in many important fields, such as in the food, pharmaceutical and cosmetic industries. The development of natural anthocyanins can meet with the consumer demand instead of synthetic colorants. Up to now, most of the research work in flowers focused on flower cultivation. How to use the natural pigment resourch is a big problem. The extraction, purification, stability and components of the anthocyanins in Impatiens hawker were studied in this paper. Main results showed as follows.1 Through the color respond solvability experiments and the analysis of spectrum data, the results indicated that the pigment of Impatiens hawker flower belongs to the anthocyanins.2 An Orthogonal experiment was designed to explore the extraction process of Impatiens hawkeri anthocyanins. The results indicated that the effect of temperature and immersing times on pigment extraction was significant, while immersing time and the value of solute/solvent was not significant. For purification method, the contrast tests were performed, and the results showed that the ion exchange resin XAD-4 method was better than HPD300, HPD600 method.3 The research has been made on stability of the anthocyanins in Impatiens hawkeri. Conclusion was drawn that the indoors nature light, temperature( lower than 80 C ), cane sugar, glucose, salt, reductant, antiseptic, K+, Na+, Ca2+, Zn2+, Mg2+ etc. have no significant effects on the stability of the anthocyanins; the ultraviolet, outdoors nature light, hydroperoxide, lemon acid, vitamin C, pH, Cu2+, Mn2+, A13+, Fe2+, Fe3+, etc. have badly destroying power to the anthocyanins.4 The high pressure liquid chromatography-mass spectrometry (LC-MS) was used to isolate anthocyanins and to determinate the molecular weight. Of those the column: Hypersil ODS C18(5 m , 3.9 150mm); solvent: (A) 0.1%TFA(concentrated TFA/water, 0.1/99.9, V/V), (B)acetonitrile. Conditions: isocratic elution at 0.3mL/min with 85%A and 15%B at room temperature; injection volume 20 L; PDA spectrophotometry, detection at 510 nm: the MS conditions: the ion source type : ESI:mass range mode : Std/Normal; ion polarity: Positive; dry temperature: 350 C; nebulizer: 35 psi; dry gas: 9.00L/min. The results showed that it conclude cyanidin-3-glucoside, may be conclude pelargonidin-3,5-diglucoside, cyanidin-3-rutinoside, Pelargonidin-3-(2G-glucosylrutinoside), Pelargonidin-3-rutinoside, and other cyanidins.