Identification Of RAPD Marker Linked To Leaf Color Trait In Cauliflower

The leaf color mutant has an important application value in breeding, For one thing, it can create the excellent resources for the species by the genie mutation, increasing the genetic variety, and select the excellent vegetable new variety and the new types; for another, the leaf mutant has a special significance and useness in nurturing the hybrid as the marker character, it can detect the mixed plant in parents and hybrid FI, and raise the hybrid pure and quality.The research used cauliflower gray-green leaf color and green leaf color mutant as the materials. The results as follows:1-. By analyzing 6 factors' amplified outcomes, a highly stable and reproducible cauliflower RAPD protocol was established. RAPD was strict with the reaction system, and the amplified outcome would be influenced by any changes of the reaction system. Therefore important factors, such as template DNA, Mg2+, Taq DNA Polymerase, dNTP, primers, annealing temperature were analyzed, On the basis of the outcome, the best system and reasonable conditions were defined, and under these conditions, RAPD bands were clear, and steady in the maps.2, By screening polymorphism between two parents with 122 RAPD 10-mer-primers, A total of 969 bands were obtained and only a band for the specific band of the gray-green leaf was a difference between two parents, the size was 1567bp.3, The S140/1567 marker was conducted a detect for the 5 single plant, and "discovered they appeared the specific band. The special band analyzed showed it was the similar band to the Arabasis chromosome 5, the similar rate was 84%; the protein was contact with Arabasis family protein, the result will benefit with research the leaf color gene control and will provide basis for the leaf color gene clone.4, The research used the green leaf mutant as the material, the mutant was rare material. The difference of the photo-synthesis was compared, the distinct was not obvious, it will not affect the photo-synthesis. To evaluate the application value of the green leaf mutant, we investigated the major economy character, the result was no difference both, which showed the green leaf character no linked to the bad character, was a rare material.5, The research reported the leaf color mutant was no reported.The innovation of the research:The RAPD protocol for applying the cauliflower was established and perfected. The RAPD marker linked to leaf color trait in Cauliflower was obtained for the first. It will be the basis of cloning the leaf color gene.