Study On Micropropagation Of Phalaenopsis Species

The genus Phalaenopsis (Orchidaceae) comprises a number of Commercially important species that have produced a wide range of attractive consumers. Phalaenopsis is a kind of popular potted-plant due to its wide variation of flower color and long florescence. In the past 20 years, the industry of Phalaenopsis in Taiwan has grown vigorously, In China, the market for Phalaenopsis has rapidly expanded too, but technique of rapid propagation lag behind.To the production of Phalaenopsis, induction and multiplication of Protocorm-like-body (PLB) are vital steps.In this experiment, top leaves of shoots cultured in darkness for two weeks were chosen that could raise the rate of induction. 6-BA is necessary for Phalaenopsis to induce PLBs, which suitable concentration is 10mg/L we have found. And, Adding adenine lOmg/L can raise induction rate too; But other factors also play important roles in induction. In the study, the different effects of NAA, myo-Inositol, Thiamine HC1, Pyridoxine HC1 and agar on protocorm formation from leaves of Phalaenopsis were compared. Experiments were done using the orthogonal design. The Results showed that: (1) NAA played the best important role in inducing. The effects of Thiamine HC1, Pyridxin HC1 and agar were stronger than myo-Inositol. (2) The best combination was NAA Img/L, myo-Inositol 200mg/L, Thiamine HC1 5mg/L, and agar 8g/L.For multiplication of PLBs, the effects of NAA, Sucrose, Maltose, Thiamine HC1, Pyridoxin HC1 were examined by orthogonal rotation combination design. The results revealed that NAA is the radical factor, and sucrose, maltose, Thamine HC1, also have important effects, but Pyridoxine HC1 is not necessary to multiplication. The optimumcombination of above factors were obtained, which is NAA 0.9mg/L, sucrose 10g/L, maltose 5g/L, Thamine HCI Img/L. The highest ratio of multiplication of PLB is 7.33 in theory.