Compositive Effects Of Band J And Two SCAR Markers On Productive Traits In New Yangzhou Chickens

DNA fingerprinting and SCAR procedures were carried with New Yangzhou chickens used as experimental animal. General Linear model (GLM) was used for the analyses of the relations between the markers and productive performance. The random primer OPAY02 was used in order to recover targeted fragments (1660bp and 2326bp, respectively). Two pair of primers were designed through cloning and sequencing procedures. The designed primers were then used for SCAR-PCR with New Yangzhou chickens DNA samples serving as the template. The results are as follows:The effects of band J on productive performance, including 8,10 and 26-week weights was analysed by DNA fingerprinting with Endogenous avian virus (EAV) used as the probe. The results demonstrated that band J exists in genome of New Yangzhou chickens and band J had significant influence on 8,10 and 26-week weights, respectively. The productive performance of the chickens without band J were higher than those with bandJ.The analyses of SCAR markers showed that S2-marker has significant effect on the three productive traits and Si-marker only has significant effect on 26-week weight Butas Si-marker is related to the other markers, it shows significant effects.In the. analyses of compositive effects, have significanteffects on the three productive traits and the optimum composite depicted as Jwas obtained.In the analyses of interactive effects, Band J, S1 and S2-markers and sex were foundwith no interaction between two factors.This investigation revealed that Band J, obtained can be used in marker assistant selection (MAS), to minimise cost, speedselection progress and stock-breeding.