Cloning And Constructiog Plant Vector For F3h Gene From Pear & Study On The Genetic Relationship Of Pyrus By RAPD

The progress of anthocyanin gene engineering was studied in Part I . A 1095bp cDNA full length sequence of f3h gene which related to anthocyanin biosynthesis was cloned. The sequence showed that it was 97% homologous identity to that of f3h cDNA from apple. The f3h gene was also introduced into pBI121 for the following research.The method of isolating genomic DNA from pear was studied in Part II. Based on the genomic DNA , It was compared that some essential factors that might affect the results of RAPD and established the optimal RAPD reaction system for pear (in a volume of 20 ul, amplification reactions system contained 2.5mM MgCl2, 200 uMdNTP, 0.2 uM random primer, 50ng genomic DNA, 1-2U Taq DNApolymerase) . Some cultivars of pear were examined by means of RAPDs. Cluster analysis based on similarity coefficients showed that Pyrus communis L. could be distinctly distinguished from P. pyrifdia Burn and P. bretschneideri Rehd . P. pyrifdia Burn and P. bretschneideri Rehd. were not clustered into different groups respectively, but there were different distance between any two accessions. We propose RAPDs as a valuable tool for the identification of specific genotypes and analysis of genetic relationships.