RAPD Marker Of Later Bolting Gene And Filtration Of Germplasm Resources On Cabbage

Cabbage is a popular kind of vegetable in the word with the characters of cold-resistant and hot-resistant. So it is widespread in china and developed fast after being introduced. But the phenomenon that crudity cabbage bolting happened frequently because climate was abnormal and cultivating patterns changed. The most economic medium solving the problem is to cultivate a variety of cabbage bolting late. This experiment used the method of bulked segregant analysis, divided bolting characters of F2 groups into two sections and established DNA pool. In order to find the gene linked together with that bolting late in the mix DNA pool by RAPD Marker analysis, further appraise the characters of resisting bolting. The main results were as follows:First, after F2 plants were treated with vernalization, the ratio of bolting was 1:2:1 among early bolting variety, medium variety and late variety by the standard of bolting time of resisting bolting and easy bolting parent material after treated with vernalization. So there may be main gene similar to quality characters despite being a quantity character.Second, through Parent C and parent F, 105 random primer were selected, and 87 primer acquired amplified products, 592 bands were gotten in all, 19 bands were polymorphism., and the good polymorphism and repeating primer with clear bands was only one, which was N1 in the two DNA poles. N1 amplified one different fragment that was N1-750, which linking cabbage bolting gene was confirmed through examining the individual plant of the two DNA poles and F2 group at each ends, and link distance was 7.9cM.Third, through RAPD method 22 materials were analyzed by selected 19 primers, only 6 primers were amplified clear and repeating bands, which got 44 polymorphism bands. through UPGMA analysis, 22 materials were categorized seven kinds according to the threshold that is the inheritance distance of 0.627. Forth, by N1-750 which is the marked linking gene. a, b, c and d were confirmed resisting bolting materials; u and v is liable bolting materials; and e, g, i, j, k and m were appraised resisting bolting materials. Postgraduate: Cao Weirong Speciality: Genetics and Breeding Supervisor: Prof.Wang Chao...