Obtained Of Chinese Cabbage-cabbage Alien Addition Lines And Sequence Analysis Of Fragment Sassociated With Bolting

Chinese cabbage (Brassica rapa1. pekinensis (Rupr.) Olsson, AA) is a type of crop with seed vernalization, by feeling cold of germinated seeds to complete the vernalization. Cultivating in spring and summer, Chinese cabbage bolt early easily and lost the commodity value and edible value of head. Head cabbage (Brassica oleracea L.v ar. Capitata1.) is a type of crop with seedling vernalization, and has a strong bolting resistance compared with Chinese cabbage. In our research group we created the monosomic alien addition lines introduced one cabbage chromosome upon Chinese cabbage chromosome background, in which one line AC4introduced Cabbage chromosome4was the most botling resistance. In this study bolting time over two years were investigated for AC4and microspore culture plants of its descendants. Plants of early bolting and bolting resistant were selected and were analyzed by using of cDNA-AFLP.The results are listed as follows:1. Using6levels of bolting standards and5levels of bolting classes, we investigated properties of bud, bolting and flowering of616microspore plants derived from different embryos of AC4and its selfing offsprings. Through2years surveying,60plants inluding45plants with bolting resistance and15plants with early bolting were obtained.2. The Chinese cabbage-head cabbage translocation lines with bolting resistance were obtained by observing the chromosome behavior and chromosome number of different plants with late bolting. The traits related to leaf, flower, middle rib, head and root of obtained plants were measured.3. Using cDNA-AFLP technology,256pairs of primer combinations were used to amplify different expression fragments between easy and late bolting plants under different vernalizations. There were124interesting bands:(1)77bands were only amplified in plants of bolting resistance, under different vernalizations14of them were expressed continuously,33of them were expressed increase,15of them were expressed decrease.(2)47bands were only amplified in plants of easy bolting, under different vernalizations8of them were expressed continuously,21of them were expressed increase,18of them were expressed decrease.2of them were expressed instant. Regulated by low temperature difference between the expression of10bands, including resistance to bolting materials strip, easy bolting materials no strip4; resistant to bolting materials no strip, easy bolting materials strip2; resistant material and easy the four were expressed.4. Ninety-five different expression fragments with clear and stable bands were cloned and Blast analysised. By using NCBI homology alignment and functional analysis, they were divided into nine categories, including7energy and metabolism genes, account for23.33%of total sequences;5genes related to transcription translation, accounting for16.67%of total sequences;4signal transduction genes, accounting for13.33%of total sequences;3membrane and transport genes, accounting for10%of total sequences;2host defense genes, accounting for6.67%of total sequences;1photosynthesis gene, accounting for3.33%of total sequences;1electron transfer gene, accounting for3.33%of total sequences;1gene related to cell walls of hydrolysis, accounting for3.33%of total sequences;1morphogenesis gene, accounting for3.33%of total sequences;5unknown function genes, accounting for16.67%of total sequences.