Genetic Analysis Of Resistance To Wheat Scab And QTL Tagging In Wangshuibai

The RIL populations of Wangshuibai /annong8455 and Wangshuibai/Alondra to wheat scab were analysised with major gene plus ploygenes. The SSR(simple sequence repeat) marker analysis and QTL(quantitative trait loci) tagging of wheat scab resistance were carried out with the population of Wangshuibai/Annong8455.The results showed as follows:the genetic analysis of Wangshuibai to scab The scab resisitance of Wangshubai fits the genetic model of two major genes plus polygenes. The RIL populations of Wangshuibai /annong8455 to scab in 2001,2003 and 2004 fits the model of E-2-6(two major genes plus polygenes with additive –epistatic effect), the model of E-1-8(two major genes plus polygenes with effect) and the model of E-1-8. The heritability of major genes is 63.8%,69.02% and 73.66%.The heritability of polygenes is 13.94%,21.38% and 16.08%. The RIL populations of Wangshuibai /Alondra to scab in 2003 fits the model of E-1-9(two major genes plus polygenes with effect).The heritability of major genes is 85.18%.The heritability of polygenes is 2.74%.the SSR marker linked to scab in the RIL population of Wangshuibai/annong845578 SSR primers have polymorphism between Wangshuibai and Annong8455 when the 236 microsatellite primers are screened. The polymorphism is 33%.There are 14 SSR markers linked to scab in the two of three experimental years(2001,2003 and 2004).They are locused on chromosome 3B,2A,2B and 5D. There are 8 SSR markers linked to scab in the RIL population of Wangshuibai /Alondra. This is published. They are at least in the two of all phenotypes and are locused on chromosome 3B,4B,2D and 4D.the tagging of QTL for Wangshuibai for wheat scab The linked map of the RIL population of Wangshuibai/annong8455 is constructed. There are 25 markers in the linked map and are locused on 5 chromosomes. There is respectively one QTL on chromosome 3B in 2001 ,2003 and 2004.The QTLs on chromosome 3B are respectively between the primer Xgwm533.1 and Barc133, the primer Xgwm389 and Xgwm533.1 and the primer Xgwm533.1 and Barc133. The LOD values are respectively 2.47, 5.57and 2.52.They can explain 10.56% , 21.97% and 8.37%of the phenotypic variations of the scab. There is respectively one QTL on chromosome2A.The QTL on chromosome 2A is both between the primer Xgwm425 and Xgwm372.The LOD values are 2.5 and 2.80.They can explain 9.76% and 11.04% of the phenotypic variations of the scab. There is one QTL on chromosome 3B in the RIL population of Wangshuibai/Alondra. It has been published.The QTL is between Barc147 and Xgwm493 and can explain 13.7 % of the phenotypic variations.The resistance to scab of Wangshuibai fits the model of two major genes and plus polygene. This helps to know the resistant to scab of Wangshuibai. The linked markers of scab-resistance is searched .There is one QTL on chromosome 3B of Wangshuibai. This is benefits to molecular breeding and is foundation to the clone of the genes to resistant to disease.