Studies On The Active Components Of Gaocha And Kudingcha

Acer.ginnala Maxim, Aceraceae, Acer.L,which belongs to defoliation little arbor or bush,is a kind of wild medical plant resource with important economic worth in forest zone of our country. It is not only a good garden and street view and admire tree , with high-quality fibre seeds.Which is the raw materials of tannin extract and black dyestuffs,but also is material resourse of Gaocha, which is a new-type health care tea beverases with drug effect of curing the disease and building up body .Kudingcha has a history of 2000 years already in our country, having much pharmacobgy effiaency .In recent years,kudingcha has been recogrized its unique quality and health care function as a kind of natural plant beverage and selled well at world ,domestic market fradually ,showing up prominently on the modern commodity market. In this thesis, Gaochas, active compositions and its function of resistsing cancer and scavenging hydroxyl free radical were preliminary discussed. At the same time, kudingcha, its function resising cancer and scavenging hydroxyl free radical were also studied. The major active components in Gaocha were studied. In analysis of amino acid , for the first time ,we found 17 free amino acids in which there were 7 essencial amino acids, 16 hydrolyzed amino acids and 7 essential amino acids among them . Extracting phenols from Gaocha with different methods, and selected the best menthods.Distilling phenols with ethanol,n-butylalcohol and acetic ether ethyl acetate.The results showed the purified phenols was 93.14 percent with the menthod of acetic ether ethyl acetate.The purified phenols was 20.22 percent with the menthod of ethanol. The purified phenols was 41.28 percent with the menthod of n-butylalcohol. The standard curve was made with a purified phenols sample, and the content of phenols was examined to be 20.06 percent. Confirmed Gaocha, quantitative method under the experiment condition, and Gaocha phenols, conversion coefficient was 2.5017. Gaocha and kudingcha phenols were Studied with spectrum analysis within the range of 190-850nm. The result indicated Gaocha phenols have two peaks of absorption whithin the range. There was a very strong absorption peak in 280nm and a very weak absorption peak in 480 nm. Kudingcha phenols have three bigger absorptions. The maxium absorption warelength was 350nm, the medium absorption wavelength was 270nm, the minimum absorption wavelength is 267nm.In the thesis, anticancer activation of Gaocha and Kudingcha was preliminarily studied, the results indicate purified Gaocha phenols perhaps have no anticancer activation, while the part of removing phenols and alkloids may have anticancer activation. So is kudingcha, remainder of removing phenols. Gaocha, Kudingcha and tea phenols obviously can scavenge hydroxyl free radical. Within the range of density designed in this experimental the scaverging rate of coach phenols increases with the increase of the density, The conversion coefficient of the two was 0.9942, when reaching 0.7 mg/ml in density, its scavenging rate was about 100%; Within the range from 0 to 0.2mg/ml in density, the higher was Kudingcha phenols, density, the higher was its scavenging rate. When reaching 0.2 mg/ml in density, its maxium scavenging rate was about 35.05% the conversion coefficient of the two was 0.9985. From 0.2mg/ml or more in density, with the increase of the density, the scavenging rate of Kudingcha phenols decline. Within the range of density from 0 to 0.4mg/ml in density, the higher was tea polyphenols, density, the higher was its scavenging rate. The conversion coefficient of the two was 0.9953, when reaching 0.5 mg/ml in density, its max scavenging rate was about 89.6%. From 0.5mg/ml or more in density, was with the increase of the density, the scavenging rate of tea polyphenols decline.