| The experiment was studied using winter wheat cultivars, Jimai20, which has high quality and strong gluten potential. The experimentation was carried out in different both fields independently at Qianzhuliu village, Beima town of Longkou city in Shandong province from 2002-2003, where available phosphorus level in 0-20cm soil layer was different, one was 15.94mg/kg, and another was 30.44mg/kg, using the method of field culture combined with 15N micro-plot. The main experimental results showed as follows. 1. Effect of amount and method of phosphorus applied on phosphorus cycle in wheat-soil system and nitrogen in soil1.1 The absorption and accumulation of phosphorus in wheat plant at different growth stage increased with phosphorus applied, however, which was reduced in the treatment of P5, P5' and split application. But the latter was right in field H-P, it has not significant effect on field M-P. In addition, treatment P5, P5' and split application have not significant difference in total phosphorus content of wheat plant.The phosphorus in 0-40 cm soil layer changed in the large extent. The total phosphorus and available phosphorus content in 0-20 cm soil layer increased, but 20-40cm decreased. The total phosphorus content in 0-20cm increased with treatment P5 in field M-P, but there was not significant effect on that in field H-P, and the content and accumulation of available phosphorus decreased. Total phosphorus and available phosphorus content and accumulation have increased in the method of split application. The totalphosphorus content in 0-20cm soil layer in field M-P increased in the treatment P5', but the content of available phosphorus and total phosphorus in field H-P in 0-20cm soil layer changed little, compared with conventional treatment.1.2 The content and accumulation of hydrolyzable nitrogen increased in 0-20 cm soil layer with phosphorus applied, but 20-40cm decreased. Total nitrogen content in 0-20 cm soil layer in field M-P and hydrolyzable nitrogen accumulation increased, but which has not changed much in field H-P with treatment P5. The content and accumulation of hydrolyzable nitrogen increased with phosphorus split application. Treatment P5' has not significant effect on hydrolyzable nitrogen and total nitrogen content in 0-20cm soil layer. The accumulation of NH4+-N and NO3-N in 0-1m soil reduced after a growth season of wheat. The accumulation of NH4+-N increased in field H-P, but little changed in another field with phosphorus applied. The content and accumulation of NO3-N increased, but NH4+-N has not changed much with treatment P5. The content and accumulation of NO3-N and NH4+-N in field H-P increased, but NH4+-N in field M-P was not affected significantly with phosphorus split application.2. Effect of amount and method of phosphorus applied on kernel quality, yield in winter wheat2.1 For field M-P, the kernel yield and quality significantly increased with phosphorus applied. Also, the corrective indexes such as weight per1000 spike grain, Farinogragh indexes etc. of yield and quality has increased correspondingly. Quality has promoted in the treatment of and but changed little in split application. On the contrary, phosphorus applied has not significant effect on the kernel yield, quality and their corrective indexes.2.2 Amylopectin, total starch and protein content in field M-P increased with phosphorus applied. Also protein content and yield increased in the treatment of P5, but which was not affected significantly in split application. Treatment P5 and split application has not significant effect on starch content. A-mylose/Amylopectin has increased with treatment P5', which was useful to improving the kernel quality. Also the protein content increased in field M-P, but there was no significant effect on field H-P.3. Effect of amount and method of phosphorus applied on carbon nitrogen metabolism in wheat3.1 Phosphorus applied treatment was useful to the accumulation and transformation of grain soluble sugar, as a result kernel starch c...
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