Studies On The Relation Between Morphogenesis And Changes Of Endogenous Hormones In Vitro Culture Of Strawberry Petiole

Studies on the relation between plantlet morphogenesis and changes of endogenous hormones in vitro culture of strawberry petiole were carried out. The result were as follows:1 There were two morphogenesis ways of the regenerate plant from strawberry petiole in vitro culture. (1) The explant petiole dedifferentiated forming callus, then the callus rediffeientiated forming shoot and root;(2) The petiole differentiated forming callus, then the callus redifferentiated forming embryoid and the embryoid grew into plantlet.2 The changes of the endogenous IAA, ABA, ZR and GA3 in the cultured materials showed following characteristics.The four hormones concentrations of the petiole increased variously during the period of callus induction. IAA, ABA and ZR promoted in induction and differentiation of the callus. It was suitable to translate 21~28d old callus into new medium for inducing adventitious bud and embryoid. It was unnecessary to add exdogeneous hormones in the new media for the callus. The multiplicationof young plant was concerned with IAA and GA3.But the low level of IAA canprompt root regeneration.3 The changes of four hormones in media showed that they came from thecultured materials. Their content increased gradually to their maximum at firstand then decreased during various culture stages. The time of appearing pinnaclewas the suitable time for the development and defferentiation of the culturedmaterials.4 Active carbon(AC) could improve the content of IAA and GA3 and reduce that of ABA and ZR in the cultured materials. Also, it could reduce the levels of all hormones in media.