The Identification Of The Dasypyrum Villosum Genome And The Study Of The Storage Protein Among Wheat Lines From The Progenies Of Dasypyrum Villosum With Triticum Aestivum

In recent years, the genetic resources of Triticum aestivum become more and more important for wheat improvement. Many alien genus of Triticum aestivum contains lots of favorable genesand possess many useful characteristics, which are the large genetic resources of wheat genetic improvement. Therefore, studying the alien gene, translating them to cultivated Triticum and increasing the genetic varieties are the important direction of Triticum aestivum breeding.Dasypyrum villosum, which is the relative genus of Triticum aestivum, is an important potential source of alien genes for wheat. So, the geneticists and breeders pay much attention to the D. villosum. Now much of work has been done, for example, the favorable genes from D. villosum were introduced into wheat genetic background through cross and backcross.In the experiment, it was studied about the genetic variations of storage protein, as well as the identification of D. villosum chromosome among wheat lines from the progenies of Dasypyrum villosum with common wheat. The results of study as the following:1. 55 gliadin bands were separated by APAGE among 151 lines and twenty to thirty bands were separated in each line. In w zone ,there are 18 bands, and r zone 17 bands .In a and B zone ,there are 10 bands expectively.The results indicated that there are abundant allelic variations at glidin loci among tested lines.2. The result of clustering by NTSYS-pc indicated that all tested lines were divided into two populations .One is MY11 population, another is D. villosum population.3. Using SDS-PAGE analysis, 8 HMW glutein subunits and 12 glutenin subunits combinations were found among tested lines. The subunit "N" has the highest frequency 59.6% at the allele Glu-Al, but "1" subunit only 38.4% which comes from the parent MY 11.This phenomenon is abnormal and further study is needed. The subunit combination N(c),7+8(b),5+10(d) has the highest frequency 27.2%.4. In this study, some near-isogenic lines (NILs) were found .406 (1, 7+8, 5+10) @ 1407 (N, 7+8, 5+10) are considered the "1 "subunit NIL. 414(2*, 7+8, 5+10)and 415(1, 7+8, 5+10)are considered the '1'subunit andthe"2*"subunit NIL;400(N,7+9,2+12)and 409 (N, 7+9, 5+10) are considered the "2+12"subunit and the "5+10"subunit NIL as well as 395(N, 7+8, 5+10) and 402 (N,7+8,2+12). These NILs would provide large of good quality and disease resistance cultivars for the breeding of wheat.5. By Gimsa-C banding analysis, several alien translocation lines were obtained. The translocation lines are 428(7VL/7AL), 480(4VL/7AL), 481(2VL/6DS). In another eight lines, D.Villosum characteristic bands are not found. Probably they are small-segment-translocation or middle segment translocation and further identification is needed.