Study On Tissue Culture Of Prunus Armeniaca Linn.

Primus armeniaca is the main sweet Prunus that spread in north China on recent years including Prunus armeniaca and so on.Now it mainly use grafting to propagation. There are a little reports about tissue culture of Prunus armeniaca in and out home. The study carried on the key factor of establishing the Prunus armeniaca no fungus system, using Prunus armeniaca stems propagate its buds and induce its callus tissue, to establish the foundation for Prunus armeniaca rapid propagation, gene keeping and genetic technique system. The detail results are as follows:1. May is the optimum time to pick explant. Using HgCl20.1% to sterilize explants twotimes, each time for 3min.2.MS medium is better than others for the beginning culture of Prunus armeniaca stems. It improved buds induce and growth.3.dded 6-BA (0.2~1.0mg/l) to propagation mediums is benefit for buds growth. When added 0.8~~1.0mg/L 6-BA, buds inductive rates is 5.9. Low concentration KT (0.1~0.8 mg/L) is no influence. When the concentration of KT is over 1.2mg/L, large callus tissue was produced.4. When N840 mg/L, K391 mg/L, C1105 mg/L in improved MS medium, buds inductive rates is highest and grow well.5. Added different concentration 6-BA and IB A in MS and improved MS mediums, it had different influence on propagation and growth. The concentration of 6-BA and IBA reach l.Omg/L and 0.08 mg/L, is best for buds propagation and growth, and improved MS medium is better than MS medium.6. When NO3~/NH4+ at 1.4 in improved MS medium, buds propagation and growth is better. NO3~/NH4+ at 0.7, increased the number of buds but decreased its growth. NO3~/NH4+ at 2.8, decreased the number of buds but increased its growth.7. Added 1.0~2.0 mg/1 GAs in improved MS mediums was clearly improved the buds growth. But the concentration of GAj over 2.0 mg/1 was no benefit for buds culture.8.The optimum leaf age for callus tissue induce was the spread young leaf. The inductive rates of callus tissue reached 87%. The colour was green and the quality is dense. Added 1.0-1.5mg/L 2,4-D or 1.5-2.0mg/L KT, the inductive rates of callus tissue was highest, and the growth was well.