Study On Diagnostic Method Of Caseous Lymphadenitis In Sheep And Goat

Caseous lymphadenitis in sheep and goat (CLA) caused by CorynebateriumPseudotuberlosis which could infect sheep, goat, camelpa, horse, bovine and so on, is akind of zoonotic and chronic infectious. Infected sheep appeared lymphondusswell,suppurative and caseous necrosis,emerging tubercles in lungs,spleen,uterus horn.Reduce of production and occur of dead fetus in infecred pregnant sheep.The occur of thedisease was slow and lethal rate low.After infection,effect of antibictic treatment to it wasnot good.So every country was increasing attaching importance to prevention theraphyexamina- tion of the disease. 2 bacteria were respectively separated from 2 sheep of Yangling's 2 sheep-farms inpreshoulder lymphonolus abscess,and identified as Corynebate- riumPseudotuberlosis,marked as A and B.16 healthy goats were respectively divided into 4groups.3 groups were respectively infected bacA bacBand standard CorynebateriumPseudotuberlosis,and 4th group as control group.100 sheep sera were collected fromYangling as samples.Before one month vaccining one goat by sterilized vaccine made bystandard Corynebaterium Pseudotuberlo- s is,we collected serum as negative serum. Weuse clinical health goat seras or somatic inhibited agglutination test negative goat cesareansection fetus seras as negative seras. Corynebaterium Pseudotuberlosis ATCC19410 was seeded into sterilized Martin-meatextract including 0.2% tween-80,cultured in 37℃.7 days later, harvested and made intoantigen of agglutination-inhibition test. Sera were collected from 4 groups,except controlgroup sera,other 3 groups sera all inhibited agglutination of the bacterium.Collectingexaminee's sera ,we did agglutination-inhibition test meanwhile setting negative andpositive contrast.If Corynebaterium Pseudotuberlosis'agg; utination were inhibited, itseemed as positive,other than as negative. Corynebaterium Pseudotuberlosis ATCC19410 was seeded into sterilized Martin-meatextract including 0.2% tween-80,cultured vibrately for 3 days, centifugaled andfiltered,made into exotoxin antigen.Selecting appropriate consistency of antigen,antibodyand enzyme-labelled,we did ELISA for examine sera and obtained positive determiningvalure,setting blank, positive and negative well.If OD valure of samples were more thanpositive determining valure,it was positive,other than it was negative. We use inhibited agglutination and ELISA examine 100 seras , results in 4 positiveseras , positive percent is 4％.