Genetic Stability Analysis And The Stress Resistance Determine Of Transgenic Tobacco

Trehalose is a nonreducing disaccharide of glucose with an a, a -1,1 glycosidic linkage. It has A special function on protection of the structures of the proteins and the membranes, as well as protect biological structures of the organisms from damage during desiccation under the abiotic stress. Thus it can made the organisms have high ability to resist drought, salt, cold and hight-temperature. So introducing and expression of trehalose synthase gene in crops can enhance their abiotic stress tolerance.In order to test the genetic stability of the transgene and analyze of their resistance to abiotic stresses, two transgenic tobacco plants and their progenies which introduced the trehalose synthase gene were used as materials to perform the molecular detection, analysis of the morphologic changes and their resistance to abiotic stress. The results showed that the trehalose synthase gene can inherit steadily in transgenic tobacco plants. Although the phenotypic alteration and slow growth occur in T0 transgenic tobacco plants, but them can restore to normal phenotype and growth in partial T1 progenies and whole T2 progenies. Trangenic plants also can synthesize and accumulate trehalose. The transgenic plants have increased their drought and salt resistance. This study has provided a good foundation for enhancing the tolerance of abiotic stresses to plants by using Tsase gene.