| The experiment studies on the impregnation rate of mature egg at different salinity and larval development at different salinity and food, and the effect weight has on individual energy budget at different salinity, temperature, Hemicentrotus pulcherrimus. Individual energy includes food consumption, excretion, respiration metabolism, fecal production and growth. The results of these studies list below:1. The experiment tests impregnation rate of mature sea urchin egg and survival rate and body length at 19, 23, 27, 31, 35, 39, 43 salinity. Within 27~35 salinity the impregnation rate can reach 95%, and it decreases significantly at other salinity, and the highest impregnation rate is almost 100% at 31 salinity. Feeding on Chaetoceros gracilis larval survival rate is 40 + 5% and body length is 789 μ m at 31 salinity, larval survival rate is 15?%, and average body length is 677 μrn at 27,35 salinity. Under other salinity, larval can' t survive through abnormality.2. Six kinds of marine microalga Chaetoceros gracilis, Platymonas spp, Phaeodactylum tricornutum, Dunaliella spp., and Dicrateria zhanjiangensis are elected as larval food to analyze the effect of food on body length and survival rate. In order to analyze the effect of food density on larval metamorphism, larval is fed with 0, 1/3, 2/3, 1, 3/2 five density level. The result shows larval food with Chaetoceros gracilis survival rate reaches 40 + 5%, body length is 789 μm, which has the best effect. Survival rate food with Phaeodactylum tricornutum is 30 + 5%, body length is 634 μ m. This experiment also indicates that Platymonas spp can be used as anaphase larval development food. Larval with other three kinds food can' t survive through metamorphism course. The larval with land 3/2 food density can complete the whole metamorphism course, other three kinds of food density level can't supply ample energy for larval development. Forepart food densityis 104cell/ml, anaphase food density is 8X104cell/ml3. Throe groups of sea urchin Hcmicentrotus pulcherrimus , weight 9.1 ?. 0, 20?.5, 31 ?.6g, are selected to study individual energy budget at 21, 26, 31, 36, 41 salinity, which includes food consumption, excretion , growth, fecal production, respiration metabolism. In order to analyze energy allocation the rate of excretion, respiration metabolism, fecal production and growth with food consumption is calculated. Within the course of 26~36, individual energy is positive direct ratio with body weight, and individual energy per weight is negative direct ratio with body weight, and both are affected significantly by body weight. Excretion is lowest at 31 salinity, and increases as salinity changes. Other individual energy reach max at 31 salinity, and decrease as the salinity add or reduce, and are affected significantly by body weight.4. Three groups of sea urchin, weight 9. l?.3g, 23?. Ig, 353?. 3g, are selected to study individual energy allocation at 8, 12, 16, 20, 24. Individual energy includes food consumption, fecal production, excretion, respiration metabolism and growth. Energy allocation includes the rate of fecal production, excretion, respiration metabolism and growth with food consumption. The result shows that individual energy metabolism bloom as the temperature increase from 8 to 20癈. When the temperature overrun 20癈, high temperature restrains energy metabolism. Individual energy is affected significantly by temperature and positive to body weight, and individual energy per weight is negative to body weight.5. Energy budget of three groups of sea urchin at 20 31 salinity: Group I 100T=17R+2U+19F+56G Group II 100T=16R+2U+20F+62G Group III 100T=14R+2U+14F+70GoGroup I : 9. l.3g. 35. 3. 3g Group II: 23. ?. 1g Group III: -36. S-L2-3g...
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