Study On Rhodiola Sachalinensis A.Bor By Callus Culture

In this paper,we studied the fittest medium of induction and differentiation on calli and culture condition of callus growth with seeds from tamed Rhodiola sachalinensis A.Bor,root,stem and leaf of tube plants and leaf of femaleand male plants in experimental field,and compared salidraside accumulation with each other.The results of experiments wereas follows:1 Good callus growth was obtained on a medium containing both NAA 2.0mg/L or 8.0mg/L,2,4-D 1.5mg/L and 6-BA 5.0mg/L or 8.0mg/L.2 Good differentiate rate was obtained on MS base medium containing both 6-BA 3.0mg/L and NAA 0.2mg/L,and good rootage rate was obtained on 1/2MS base medium containing IAA 1 .0mg/L.3, Good callus growth and salidroside accumulation were cultured under 24 and12-14h.4, Sucrose had significant effects on the accumulation of salidroside and MS medium containing 3.5% sucrose was the best.5, Salidroside accumulation of calli inducted from leaves of female and male had more significant difference,and male was more than female.