Relative Bioavailability For Different Organic Manganese And Iron Sources In Broiler Chicks

Two experiments were respectively conducted with 200 one day-old Avian broiler chicks to estimate bioavailability of different iron sources and manganese sources for broiler chicks. factorial design was used in both experiments. Broiler chicks were allotted randomly to ten dietary treatments with four duplicates that included an unsupplemental corn-soybean basal diet (44mg/kg Mn or 94mg/kg Fe) or this basal diet supplemented with 60, 120 or 180mg/kg Mn either as MnSO4 H2O, manganese acetate or yeast manganese in the first experiment, or this basal diet supplemental with 40, 80 or 160mg/kg Fe either as FeSO4 7H2O, ferric citrate or yeast iron in the second experiment. After having been fed manganese-deficient or iron-deficient basal diet for 2 days, Chicks were fed experimental diets for 21 days. Chicks were killed and tibia, kidney, muscle and liver, hemoglobin, and MnSOD were determined in the experiment of manganese, or spleen , kidney, pancreas and liver Fe concentration, hemoglobin, TIBC, RBC and HCT were determined in the experiment of iron.In the experiment of manganese the results indicated that: Under the conditions of this experiment, performance, liver, muscle, kidney, bone Mn concentration and MnSOD were not affected by dietary Mn, but hemoglobin were significantly affected by dietary Mn. Under the conditions of this experiment, with increasing dietary Mn, F/G decreased, but liver, muscle, kidney Mn concentration significantly increased, hemoglobin signigicantly decreased. Under the conditions of this experiment, using the slope-ratio technique from the value of different indexes on mean daily Mn intake, relative bioavailability of manganese acetate or yeast manganese was different.Synthetically analysis of the significance of regression equation and the effect of dietary Mn on indexes determined in this study showed that all determined were sensitive indicators.In the experiment of iron the results indicated that: Under the conditions of this experiment, performance, spleen Fe concentration and hemoglobin, RBC, HCT, TIBC and thymus index were not affected by dietary Fe, but liver Fe, kidney Fe concentration and pancreas Fe were significantly affected by dietary Fe. Under the conditions of this experiment, with increasing Fe dietary, performance and thymus index did not increase or decrease, but spleen and pancreas Fe concentration and hemoglobin significantly increased, TIBC signigicantly decreased. Under the conditions of this experiment, using the slope-ratio technique from the value of different indexes on mean daily Fe intake, relative bioavailability of ferric citrate or yeast iron was different. Synthetically analysis of the significance of regression equation and the effect of dietary Fe on indexes determined in this study showed that all determined were sensitive indicators.