Studies On The Relationship Between Polymorphim Of Povcine Pit-1 Gene And Their Reproductive Performance

Reproductive performance are important traits in Swine breeding and production. The identification of Candidate gene associated with reproductive is an effecitive messure to improve productivity. It will be very important on theoretical & Practical swine breeding and productive. Pit-1, as one important member of the POU-domain family of genes , is a postive regulatory factor of GH, PRL and TSH B .In mammals, it is believed that Pit-1 play a very important role in animal reproductive and growth.In this Study., The Polymorphisms of porcine Pit-1 partial region in four breeds pigs including Erhualian, Meishan, landrace and Yorkshare were investigated. A pair of primers were used to amplify a 1746 bp DNA fragment which started from exon5 to the end of exon6, then two enzymes (Pst I and Rsa I) were used to digest this PCR product respectively and detected it's polymorphism. We found that Pst I enzyme produced four fragments (811bp 570bp 546bp and 360bp) and three genotypies(AA, AB, BB), while Rsa I enzyme produced 736bp and 710bp or (388bp and 322bp) fragments and three genotypies including EE, EF, FF. We got a fact that a 24bp DNA fragment deletion in Pst I enzyme restrict site.Also we analysed the difference of allele genotypes in four breeds pigs and their correlation with reproductive performance. X2--Test showed there are signtificant difference between Yorkshare and Erhualian. China local breed Erhualian's B allele frequence was signtificant higher than Yorkshire. Former's TNB Significant different to latters, and Erhualian sow's birth little weight and weaning litter weight were significant higher than Erhualian gilts's (0.01