Detection And Analysis On Pathogenetic Nucleic Acids Of 3 Viral Infections And Serum Antibody Levels Among The Farmed Fur Animals In Vaccinated Status

Canine Distemper and Canine Parvovirus Enteritis are the two most important viral infections commonly occur among farmed fur animals, while Fox Encephalitis is a kind of viral infection among foxes. It has been decades since the prevalence of these three kinds of infections among fur animals. They have occurred in almost every raising area and farm throughout history. All of these three infections occur and develop at a high speed. Once they prevail, it will be followed by high incidence rate and mortality. A great number of deaths can not only lead to severe economic loss for the owners, but also badly influence and restrict the development of the whole industry. Recently these infections are well prevented and better controlled than before, however, they still occur and prevail every year because of various of reasons. In order to prevent and control these infections, efficient, simple, rapid, reliable and specific diagnosis methods are required. Moreover, we should comprehend the occurrence and prevalent trend beforehand.In this study, we (1) sampled routine vaccinated animals from some farms which raise more than 3000fur animals in Shan Dong and Hei Longjinang provinces ect. (2) optimized the PCR protocol for detection and diagnosis, and at the same time give specific detection on nucleic acid in CDV and Fox Encephalitis virus from samples collected from vaccinated animals. (3) applied immunoenzymatic assay to detect and analyze serum antibodies titer in CDV and Fox Encephalitis virus; while applying HA and HI to detect and analyze serum antibodies titer in CPV. (4) analyzed antibody levels, comprehend effects of immunity, and analyze reasons of failure for immunity. Connecting prevalent conditions in recent years to prevalent history, try to analyze occurrence, development and prevalent reasons and trend of these three infections.