Study On Construction Of RS Gene Expression Vectors And Genetic Transformation System Of Saccharum Officinarum L.

Resveratrol is a kind of important phytoalexin that has many functions for health and medical treatments,so its extensive application prospect has already caused attention in many ways.Resveratrol synthase is an exclusive necessary enzyme in the pathway of resveratrol biosynthesis.which catalyzes one molecule of 4-coumaroyl-CoA and three molecules of malonyl-CoA into resveratrol.The research about it has extensively developed.Saccharum officinarum L. is a high output crop. There are many problems in Saccharum officinarum L. industry at present such as the quality, the serious diseases and insect pests etc.Some useful foreign genes could be transferred into Saccharum officinarum L.by engetic engineering to improve Saccharum officinarum L.varieties and enhance the economical value. It may has broad application perspective if Saccharum officinarum L.could be served as bio-reactor to produce some medical proteins.The present study is for resveratrol synthase gene cloning from peanut. Both monocotyledon and yeast expression vectors were constructed in the study. Meanwhile, the regeneration system of Saccharum officinarum L. was optimized. Resveratrol synthase gene transforming into the embryogenic callus of Saccharum officinarum L. was tested.The results were as follows:1. The monocotyledon expression vector including Ubi promoter was constructed.2. The yeast expressin vector was constructed.3. The regeneration system of Saccharum officinarum L. was optimized. The optimized medium in the induction phase is MS+2.4-D 2mg/L; The optimized medium in the regeneration phase is MS+NAA 0.2mg/L+BA 2mg/L+ Activated charcoal 0.5mg/L; The optimized medium in the root growth phase is 1/2MS+NAA 2mg/L Activated charcoal 0.5mg/L.4. Preliminary resistant screening experiment was taked to the embryogenic callus of Saccharum officinarum L. by G418. The screening concentration in the regeneration phase is 30mg/L; The screening concentration in the root growth phase is 25mg/L.5. Resveratrol synthase gene transforming into the embryogenic callus of Saccharum officinarum L. was tested. In the present, the G418 resistant regenerated seedlings were obtained.