Studies On Cloning Of Pig ADD1 Gene And Its Relationship With Meat Quality

People will require high quality pork with ceaseless improve of live level. It is doubtless that improve meat quality is emphases in animal breeds in the future. In human research, there is doubtless association between adipocyte determination and differentiation factor (ADD1) gene and aggradation of fat out of the non-fat tissue. ADD 1 gene, PPAR gene and CEBP gene regulate corporately process of adipocyte differentiation. AT the same time, ADD1 gene regulate expression of LDLR gene.ACC gene.FAS gene, GK gene, PEPCK gene etc, which have important role in adipocyte synthesis and metabolism of glucose. Recently research show, ADD1 gene can regulate fat aggradation in non-fat tissue. Therefore, we research the association between ADD1 gene SNP of ADD 1 gene and meat quality as a candidate gene. The works and conclusions show:(1) Cloning of ADD1 geneIt is first time that cloning porcine complete ADD1 cDNA sequence and partial sequence DNA. We can identify the sequence with analysis of homogeneous, conservative structure of protein three structure, Southern bloting. The cDNA sequence has submitted to GenBank (Accession No. AY496867), The DNA sequence has submitted to GenBank (Accession No. AY272051).(2) Expression of ADD1 geneWe have analysis of ADD 1 gene expression in ten tissues of heart, liver, spleen, lung, kidney, stomach, large intestine, small intestine, and muscle, fat, with half ration RT-PCR. The result show all tissue have expression of ADD1 gene except for liver and the highest expression in fat tissue, the lowest expression in muscle tissue. All of this is theory of basic of association between meat qualities with ADD1 gene.(3) Analysis between the SNP of ADD1 gene with meat qualitySNP have been found after scan partial DNA sequence. We have detected different genotypes in two groups, different feed factor and different breed factor, with PCE-SSCP. We have designed different modules in different groups to analysis association between SNP with meat quality. The result in A group show, AB genotype and BB genotype have significant difference (P<0.05)in color and intramuscule, AB genotype has higher intramuscule and lighter meat color, different feed factor have very significant difference(P<0.01) in meat color, intramuscule, protein, pH value. The result of B group show, There is between significant difference (P<0.05)in meat color, intramuscule, tendness, and BB genotype has higher intramuscule and tendness, lighter meat color. Different breed factor have very significant difference(P<0.01) in all index. In conclusion, there is association between the SNP of ADD 1 and porcine meat quality, and ADD1 gene is major gene or association with major gene. We have only found a locus of proteolysis enzyme with biologic softwareanalysis RNA second structure and third structure, isoelectric point, transmembrane segments, locus of proteolysis enzyme. It is conceivable reason of association between the SNP of ADD1 and porcine meat quality.