| The present study chosen female animal model with cadmium loading via diet or drinking water, and employed modern biological techniques in order to systematically observe disruptions of function activity of some important immunologic cells, plasma immune-mediators level and reproduction endocrine function in female rats induced by cadmium loading, meanwhile to investigate effects of cadmium on growth, nutrient metabolism, plasma hormone level, hepatic IGF-1 mRNA expression and zinc concentration in maternal rats and their offspring. The results were summarized as follows:1 Changes of function activity of immunologic cells and some immune mediators in female rats with chronic cadmium loadingSeventy-two healthy Sprague-Dawley female rats were chosen and divided into a control group(group C), a cadmium loading group of low dose(group L-Cd), a cadmium loading group of medium dose(group M-Cd) and a cadmium loading group of high dose(group H-Cd). Groups C, L-Cd, M-Cd and H-Cd were orally dosed daily with 0, 2, 5, 10 mg of cadmium per kg feed over 9 weeks, respectively. Body weight three groups with cadmium loading was lower or significantly lower than group C; T-lymphocyte(%) reduced, and groups M-Cd and H-Cd decreased significantly as compared with group C, meanwhile, B-lymphocyte had opposite changes; RBC-CR1 rosette rates(%) of all groups treated by cadmium were low or significantly low to that of group C during the whole experiment period, while RBC-IC rosette rates(%) were also lower than group C but there were no significant differences; stimulate index of spleen lymphocyte transformation were significantly lower than that in group C; spleen NK cell activity decreased, with a markedly difference between group H-Cd and group C; Plasma IL-2 were low to group C but no significant differences were observed; TNF-# level was superior to group C in the 3rd and 9th week, respectively, and group M-Cd was significantly higher than group C in the 9th week; Plasma cAMP of groups L-Cd, M-Cd and H-Cd were higher than that of group C, and groups M-Cd and H-Cd in the 3rd week as well as group L-Cd in the 9th week were markedly superior to group C; plasma cGMP of L-Cd and M-Cd were significantly low to group C in the 3 rd week; the ratio of cAMP/cGMP of all cadmium-loading groups were higher than group C during the whole experiment period. It was suggested that chronic cadmium loading can cause the defect of immune cell function in female rats, and changes or disorder of some immune-mediators may play a action in participating in the cadmiun-induced immunosuppression.2 Effects on phagocytic function activity of macrophages in female mouse with chronic cadmium loadingSeventy-two healthy female ICR mice were chosen and divided randomly into four groups including into a control group(group C), a cadmium loading group of low dose(group L-Cd), a cadmium loading group of medium dose(group M-Cd) and a cadmium loading group of high dose(group H-Cd), which were orally dosed daily with 0, 2, 5, 10 mg of cadmium per kg feed over 9 weeks, respectively. There was no significant difference though an increase tendency in body weight was observed with age of week among every groups. Phagocytic rates in macrophages of groups L-Cd, M-Cd and H-Cd reduced significantly as compared with that of group C; however, phagocytic indice also reduced but no obvious differences were observed. There were no obvious differences of plasma TNF-a among all groups. It suggested that chronic cadmium loading induced the defect of macrophage function activity in mice .3 Effect of chronic cadmium loading on reproduction endocrine function in female ratsSeventy-two healthy Sprague-Dawley female rats were chosen and divided into a groups C, L-Cd, M-Cd and H-Cd, and four groups were orally dosed daily with 0, 2, 5, 10 mg of cadmium per kg feed over 9 weeks, respectively. Plasma etradiol-l7 level was close to each other, so were progesteme and cortisol level. No obvious difference was observed during the entire experimental period. It seems that... |
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