Capacitation And Fertilization Of Boer Goat Sperm In Vitro

Objectives of this study were to select a disirable culture system in vitro capacitation and fertilization of Boer goat spermatozoa.Most goat spermatozoa displayed hyperactinated motility after incubated for 4h.Sperm was treated with two methods and three culture media, capacited sperm exposured to lysophosphatidy-choline(LC) to induce acrosome reaction. Capacitation was evaluated by determining the number of motile acrosome-reacted sperm, Sperm was incubated in T media which was added heparin or Ionophore A23187.Results indicated swim up is better than centrifugation. The capacitation rate of goat sperms incubated for 4~6h was significantly higher(p<0.01) than that incubated for less than 4h.T media was the best culture media allowing for its effects on capacitation and motility of sperm.The percentage of acrosome-reacted sperm at 4h was significant difference from the other media(p<0.01). Supplements of 20ug/ml or 50ug/ml heparin had apparent effect on acrosome reaction of sperm(p<0.01). But there was no apparent difference between two media(p>0.05). The dosage of IA is suitable on 0.3umol/L.After addition of capacited sperms treated with heparin or IA to the hamster eggs. Penetration rate of sperms incubated for 4~6h was significantly higher than that for 2h.Penetration rate of goat sperms treated with 20ug/ml heparin or 0.3umol/L IA was 64.7% or 68.2% respectively. Caffeine had a synergetic effect in combination withheparin or IA on enhancement of penetration rate.The higher fertilization rate was obtained when oocytes and sperm cells were incubated for 17h than 8h(P<0.01).The fertilization rate of IVM oocytes was 60.0%, and fertilization rate of oocytes after superovulation was 65.0%,the latter was little higher.Because of relative fragility of goat sperms after freeze-thawing, Its viability rapidly decreased after treatment of in vitro capacitation. Acrosome reaction rate indicated fresh sperm was significantly higher than freeze-thawing sperm(P<0.01).