Studies On The Ingredients Of Diluent Of Boer Goat Frozen Semen

The aim of this study was to improve the quality of Boer goat frozen semen, making to arrive the level of the Boer goat fresh semen. In this study, The ingredient of diluent was finished at the base of the four finest twice diluted ingredients of Boer goat frozen semen diluted ingredient of Boer goat frozen semen diluent. The analyse of the goat spermatozoa ultrastructures, measuring the active of GOT-released and LDH-released, and the ability of penetration were investigated respectively. The results were following:1. In treatment IV (pH7.2), after frozen-thawed, the active of spermatozoa , recovery were respectively: 0.489 0.011, 69.6 1.535. But in treatment I (pH6.6), It was: 0.411 0.006 , 59.7 0.317, there were a very significant difference (p<0.01), and had a significant difference (p<0.05) with treatment II (pH6.8) , about the rate of integral top, treatment IV (pH7.2) was 68.2 3.129, which had a significant difference (p<0.05) with treatment I (pH6.6),treament II (pH6.8). The result indicated that the effect of treatment IV (pH7.2) was the best.2. In treatment IV ( 0.819 ), after frozen-thawed, the percentages of spermatozoa 0.478 0.012, the rate of integral top 68.2 3.129, these were all elevated very remarkably (p<0.01), comparing with treatment I ( A 0.526 ); there were a significant difference (p<0.05) between treatment IV (A 0.819 ) and treatment II (A 0.682 ), treatment III( A 0.754 ). It shown the higher infiltrate pressure was favorable of the conservation of Boer goat frozen semen.3. In treatment II, which contains 4% glycerin, the percentages of spermatozoa motility, recovery were respectively: 0.495 0.011, 65.066 0.216., There were a significant difference with treatment 1(2.4%), treatment III (5.6%), treatment IV (7.2%) and treatment V (8.8%) (P<0.05) This explains that the fine concentration of glycerin had an effect on the quality of the goat frozen semen, also the effect of 4% is the finest in the study.4. In the ingredient V, after frozen-thawed active , the survive time, the active of frozen-thawed conserving (8h) , the rate of integral top were consequently: 0.492 0.012, 11.616 0.117% 0.381 0.012, 69.866 1.535,these were reduced remarkably between I , II, III., IV(P<0.05). The result indicated that the comprehensive effect on using the alleviant of Tris and citrate was better.5. Ingredient V, the leakage of GOT and LDH were reduced remarkably(P<0.05), comparing with the ingredient I , ingredient II, ingredientlll, the active of GOT and LDH were respectively:268.1 1.820 , 85.1 0.258,It is important that sugar had several functions in the diluent, as the nutrient , protection and alleviant.6. SPA.in the ingredient V, the ability of penetration was83.194 2.164,there was a very significant difference(P < 0.01) with ingredient I , III .and had a significant difference(P<0.05). It shown ingredient V was the best of these ingredients on the ability of fertilization.