Effect Of Hyaluronic Acid On In Vitro Maturation Of Oocytes, In Vitro Culture And Freezing Conservation Of Embryo On Bovine

In this study, hyaluronic acid (HA) was added to the modified foundation medium , throughwhich to mainly determine the effect of HA on in vitro maturation (IVM) of bovine oocytes,in vitro fertilittation ( IVF), in vitro culture (IVC) Freezing Conservation (FC).The resultshow that: 1. the group with 4mg/mL HAwas significant higher than other groups in the frequencyoffirst polar body extrusion (PB) and cleavage rate of oocytes(P<0.05), this primitivelyexpresses that 4 mg/mL was the best concentration for free serum culture with HA. 2. When we added HA , bovine serum albumin (BSA)or Oestrus Calf Serum(OCS )toTCM-199, the group with the OCS was significantly higher than other two groups (83.40%,84.33% and 92.17%, P<0.05) in PB, but cleavage rate was no significant different in threegroups P>0.05; there were no different in PB of BSA and HA (P>0.05).Thereof HA was asubstitute for bovine serum albumin(BSA)in serum-free medium to mature the oocytes,butthe effect was not equal to blood serum in result of the culture. 3.HA in TCM-199 and Synthetic Oviduct Fluid (SOF) have no effect on in vitromaturation of bovine oocytes in PB of oocytes and cleavage rate; it demonstrated that HA canfavor the maturity ofoocytes in complicated or simple free-serum medium. 4. It is important the effect of the concentration of HA on bovine earlier embryodevelopment, to or not add HA is significant different (P<0.05) between groups; whenconcentration of HA is 2~4 g/L, there is 31.76% of blastocyst rate, which show 2~4 g/L is thebest concentration. 5. In different additives, the group with HA demonstrated significantly higher than groupwith polyvinyl- alcohol (PVA)(respectively for 31.54% with 26.61%,P＜0.05)in blastocystrate,but lower group with FCS (P＜0.05); it was no significant different with BSA group (P＞0.05) in blastocyst rate; Those which show BSA will be best replaced by HA. 6. In free-serum medium, oocytes matured with HA is significantly higher than PVA inblastocyst rate (respectively for 32.35% with 26.80%, P<0.05); However, it is significantlylower than ECS (respectively for 32.35% with 34.91%, P<0.05); Although it is higher thanBSA there is no significantly different in them (P>0.05). 7. Although Synthetic Oviduct Fluid (SOF) is higher than TCM-199 there is nosignificantly different in them (respectively for 31.03% with 30.43%, P>0.05), which showHA can all improve bovine embryo development in SOF and TCM-199. 8. Embryo cultured by TCM-199+ HA was significant higher than group that did not addHA (P＜0.05) in its survive rate and hatch rate (respectively for 65.85% vs 58.69% and60.98% vs 36.96%) after freezing-thawed. 9. Embryo cultured by SOF+ HA was significant higher than group that did not add HA (P＜0.05) in its survive rate and hatch rate (respectively for 58.33% vs 43.14% with 52.08 vs31.37%) after freezing-thawed. 10.Embryo cultured by TCM-199+ HA was significant higher than SOF+HA in survive rateand hatch rate (P＜0.05) after freezing-thawed.