Studies On Effect Of Mycorrhizal Fungi On Promoting The Resistence To Adversity

The experiment study the anti-adversity of Pinus tabulaeformis and Forsythia suspense (Thunb.)Vahl by research of resource ,cultivation of ectomycorrhizal fungus ,the test of resistence and incolation. The results are as follow:The result of Ectomycorrhizal fungus resource show ten species of six genus can be picked up mushroom in pure P.tabulaeformis forestry , Luolan and Danfeny ,shannxi province.The ten fungi include Suillus grevillei(Klotzsch)Sing, Scleroderma polyrhizum Pers.,Lepiota procera S. F. Gray., Cortinarius sp.,Suillus bovines L.:Fr.,Leccinum scabrum(Fr.)S.F.Gray.,Cortinarius multiformis (Fr.) Fr,Scleroderma areolatum Ehrenb.and Scleroderma areolatum Ehrenb., Cortinarius gentilis Fr.;Three symbiotic fungi spore of Forsythia suspense was found in soil by the root of Forsythia suspense, they are Glomus constrictum Trappe, Glomus fasciculatu Gerd. & Trappe emend. Walker & Koske and Gigaspora gigantean Gerd. & Trappe, G. constrictum has mumber superiority. The five Ectomycorrhizal fungi of S.polyrhizum,L. procera,C.gentilis,C. sp and S. bovines were departed from the root of P. tabulaeformis and mushroom. Boletus edulis Bull.:Fr and Suillus luteus L.:Fr are provided by microbe lab. The seven fungi were cultivated on different culture,The results show all the fungi except L. procera adapt to cultivation in nature culture. The others except Cortinarius can be cultivated in MMN liquid culture.S.luteus,B.edulis and C.gentilis are better than the others.S. luteus has remarkable resistance to the two kinds of pathogen, next to B.edulis,S. bovines and L. procera. That experiment showes the summit activity of POD,CAT and SOD in P.tabulaeformis inoculated by ectomycorrhizal before pathogenic fungi. The activity inoculated by ectomycorrhizal fungi is equal to the pathogen. It indicates the inoculation by ectomycorrhizal fungus would enhance the activity of defense enzyme under the force of drought. The resistance of the plant to disease was strengthened. S. bovine. B.edulis and L. procera have better effect.The experiment about the drought-resistence of Pinus tabulaeformis test the four kinds of the physiological indexes of drought-resistence in P.tabulaeformis inoculated by the seven kinds of mycorrhizal fungi in liquid and solid culture .The results show liquid mycorrhizal fungus were superior to the same solid;All mycorrhizal fungis can enhence the resistence of Pinus tabulaeformis to drought, B. edulis has best effect,next to C.gentilis Fr.The rate of death reduces under drought after F.suspense was inoculated by G.fasciculatum, G.constrictum and two kinds of mixture, at the same time, measure chlorophyll, proline , POD and so on, the result show mycorrhiza fungi can pass to change the content of the chemistry composition inside the plant body after invading, defering the plant to suffer from the harmful speed, strengthenning the anti- drought of the plant, among the all inoculation, the result of G.constrictum is better.