The Expression Of IL-1b In The Uterus And Placenta Of Pregnancy Rat And Its Regulation By Th1 Type Cytokines

In the study, Spreqne-Dawley rat was chosen as in vivo model to examine interleukin-1beta (IL-1β) expression level in uterus and placenta in the different phase of pregnant and the effects of interferon-gamma(IFN-γ) and IL-1β on the expression of IL-1β in uterus and placenta using RT-PCR and immune histochemitry. The results were as follows:(1)In the course of pregnancy, uterus and embryo expressed IL-1β, which changed according to time and order. Maternal decidual cell had a little IL-1β in early gestation. Decidual rebuilding vein and maternal-embryo interface expressed more IL-1β and part syncytiotrophoblast also expressed more IL-1β in mid gestation. Decidual rebuilding vein and trophalast cell including syncytiotrophoblast and cytotrophoblast expressed some IL-1β. (2)IL-1β expression in uterus was significantly reduced in peri-implantation,post- implantation,mid and late gestation period, and IL-1β expression in placenta was significantly decreased in late gestation period after injecting over-dose IFN-γ. This indicated over-dose IFN-β could suppress the expression of IL-1β of uterus and placenta in certain pregnant phase.(3)IL-1β expression in uterus was significantly increased in peri-implantation,post- implantation and mid gestation period, and IL-1β expression in placenta was significantly increased in early and late gestation period when injected over-dose IL-1β. This indicated IL-1β could prompt the expression of IL-1β of uterus and placenta in certain pregnant phase.(4)IFN-γ restrained development of embryo and placenta through decreasing the expression of IL-1β, and induced abortion and premature. The effect of IL-1β on development of embryo and placenta was different in the different phase of pregnancy.In summary, the expression of IL-1β in uterus showed evident trends, and in placenta had little change. In certain pregnant phase, IFN-γ could suppress the expression of IL-1β of uterus and placenta, while IL-1β could prompt the expression of IL-1β. This shows Th1 type cytokines are always not to induce the expression of homotype cytokines. Those may be concerned with stabilization of in vivo circumstance in the course of normal pregnancy.Postgraduate:...