Morphology Study Of Sperm-host Glands In The Oviduct Of Avian

This experiment regards fertile and infertile hens, ducks, geese and quails as experimental animals, and adopted the method of general anatomy, histology, histochemistry and electron microscope technology, combined function of oviduct to study morphology of uterovaginal sperm gland.Result of anatomy observed indicated: sperm gland of avian is single cannular gland in the immanence layer of mucous membrane that is encircled by single columnar epithelium and subulate cells, the length of oviduct and the width of orbicular mucous membrane that contained sperm glands is different for the diverse function of oviduct and species, the sperm glands is the more fertile than infertile, the diameter of gland cavity is changing with function of oviduct.Result of histochemistry observed indicated:the sperm glandular cells of hens, quails and geese represented positive response by PAS, but ducks' reprexented PASnegative response in the fertile and the infertile, the sperm glandular cells of hens reprexented positive response by fattiness dyed,but ducks', geese' and quails' reprexented negative response in the fertile and the infertile.Result of ultrastructure observed is that we can see that the sperm glandular cells is single columnar or subulate epithelium cells, karyon is circular or elliptic, and lied in the fundus of cells, nucleolus is clear, microvillus of cells dissociative sides is developed, developed interdigitation and plasma membrane infolding that was observed peculiar structure lied in the profile and the fundus, and can see incompact developed virgulate mitochondria that lied in the cytoplasm, rough endoplasmic reticulum is not developed, lysosome is exiguous, hepatin granule distribute symmetrically except hens in the cytoplasm, circular vacuole structure is observedabove the sperm glands karyon of hens and under the sperm glands karyon of geese, but this structure can not find in the sperm glands of quails and ducks, longitudinal arranging sperms are contained the sperm glandular cavity.In this experiment, we work for the morphology study of three different stages of chickens, ducks, goose and quails, and probe into the variety of shape and configuration of the sperm gland cells, and summarized changing disciplinarian of histology, histochemistry and ultrastructure of the sperm gland cells, provided theoretic gist of morphology to expound physiological mechanism that the sperm gland storage sperm.