| Sepiella maindroni is an important marine cephalopods economic animal, which belongs to Mollusca,Cephalopod, Decapoda, Sepia esculenta. In this paper the digestive system is researched by relatedanatomical, histological, cytological and enzyme chemistrical. The results are as follows:Through the anatomical, histological and histochemical methods research shows: Sepiella maindronidigestive system consists of a port ball, esophagus, ball salivary gland, liver, pancreas, stomach, intestine,caecum and ink. The port ball has proboscis, which is respectively through the muscles and connected withthe inner walls of the esophagus. The esophagus folds more and the inner wall of ciliated cells are rich. Apair of enlargement of the salivary glands which is central in the esophageal is near the liver, like soybeans.The stomach is nearlyround, a type of D. The gastric wall muscular layer is very thick and ciliated cells arerich. Intestinal is small, divided rectum and intestine. Its bowel wall is composed of mucous layer,submucosa layer and muscle layer. The mucosal layer is mainly composed of columnar epithelial cells andsubmucosal muscle fiber is rich, but less capillaries. The muscle layer is thinner. Liver contains numeroushepatic lobules and each lobule is composed of the the outer edge gland cells and the surrounded germ cells.Pancreas has a lot of yellow small particles, whose main cells are the embryo cell and the secretory cell.Blind sac is located in back end of stomach, in which there are crests spanning around. The main types ofcells are goblet cells. The ink sac digestive function degrades. The membrane of esophagus, pancreas, liver,blind sac and intestinal has protein. Caecum, intestine, rectum and esophagus mucous cell cytoplasm,salivary gland, liver and pancreatic secretion of the cell cytoplasm and cuticle contain moremucopolysaccharide.By observation ultrastructure of adult Sepiella maindroni digestive system using transmission electronmicroscope, It shows that the Sepiella maindroni Esophagus Cells have secretory cells, epithelial mucouscells and a few phagocytic cells. Secretory cells have abundant organelles, muscle fibers, usually formonocytes, occasionally binucleate cells. Salivary glands are only observed in salivary gland secretorycells and its membrane system is developed. The stomach is found only in gastric secretory cells whosemitochondria are very rich, and there appeared special mitochondrial clusters called mitochondrial vesicle.Binucleated cells are much. Caecum mainly has absorption cells, microvilli cells, secretory cells. The mostremarkable characteristic is cup-shaped absorption cells which are arranged in a line Secretory cells are fullof lipid droplets and obvious glycogen particles. Intestine mainly has intestinal epithelial mucous cells,microvilli cells, absorptive cells and secretory cells. There are abundant lipid and carbohydrate in cells.Single nucleoli and in two nucleoli are easy to see, even three nucleoli. Organelles are rich, particularly theendoplasmic reticulum which is particular developed, and also appeared a closed circle of endoplasmicreticulum. Pancreatic cells mainly have secretory cells, ciliated cells, secretory cells. The secretory cells arethe richest and there are abundant mitochondria, endoplasmic reticulum and lipid droplets in it. We can seemore binucleate cells.In this study, we make enzymatic analysis of main enzyme on adult and larval Sepiella maindronistomach, caecum, intestines, pancreas and liver five digestive organs. Pepsin activity, trypsin, amylase, andlipase activity four key enzymes and compared the differences between them. The results showed that:adult pepsin activity are as follows: stomach (6.0714)> liver (2.6291)> pancreas (0.8859)> caecum(0.2995)> intestine (0.1288), larval pepsin activity are as follows: stomach (5.3088)> liver (2.5339)>pancreas (1.0315)> caecum (0.2984)> intestine (0.1495); adult trypsin activity are as follows: pancreatic(1138.3529)> caecum (1351.4501)> stomach (403.6705)> intestine (261.8600)> liver (212.3160), larvaetrypsin activity are as follows: caecum (1117.0775)> pancreas (1103.8025)> intestine (256.6668)> stomach(220.8994)> liver (149.6912); adult amylase activity are as follows: pancreas (0.1232)> liver (0.0905)>caecum (0.0752)> stomach (0.0355)> intestine (0.0073), larval amylase activity are as follows: pancreas (0.1233)> caecum (0.1088)> liver (0.1007)> stomach (0.0462)> intestine (.0119); adult lipase activity areas follows: liver (274.6931)> caecum (79.0587)> stomach (53.1819)> intestine (14.8246)> pancreas(11.4550), larvae lipase activity are as follows: liver (102.0174)> stomach (41.5069)> caecum (38.4770)>pancreas (9.5125)> intestine (1.9724). Trypsin in adult stomach is about1.8times as the larvae and lipasein the adult body is about2times as larval. Lipase in adult liver is approximately2.7times ae the larvaeand amylase in the larvae blind sac is about1.5times as the adult. |
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