| The study was conducted with three experiments (experiments in vitro, in weaned piglets, in broilers) in Animal Nutrition Department Institute of Animal Science of CAAS (Chinese Academy of Agricultural Sciences) to study the effects of inulin on intestines enviromental in monogastric animal. It's proved that inulin could improve health of animal and boost animal growth. Thereby inulin is a safety, effective and green feed additive.Experiment one was isolated culture. Six treatments and six replicates conducted. The six treatments were 0g/L, 1 g/L, 2 g/L, 4 g/L, 6 g/L and 8 g/L inulin group respectively, and the basic culture medium of these treatments was same. 4 grams fresh pig feces were cultured by anaerobic fermentation. And pH and content of VFA were determined after 24 hours, to investigate the effect of inulin on microbe.Experiment two was feeding trial of weaned piglets. 28-day-wean six piglets, with randombody weight, were randomly allotted into five treatments with three replicates in each treatment. Different content of inulin was put in the basic diet, the basic diet of the five group was same. The experiment involved 4 dietary levels of inulin (the antibiotic groups within 0%, 0.5%, 1%. 1.5% inulin respectively, and 1% inulin group without antibiotic). On the last day of this experiment phase. 12 piglets from 3 treatment (0% inulin group, 1% inuin group, and 1% inulin group without antibiotic) were taken out randomly, stripped heart then slaughtered, hindgut samples were collected to determine content of VFA in cecum, the microflora population in cecum and colon, pH in intestines and feces. apparent digestibility of calcium, activty of inulinase, and cholesterol in serum.Experiment three was feeding trial of broilers. 120 21-day-old Arbor Acre (AA) broiler chicks were randomly allocated into 4 groups, each with 6 replicates of 5 birds. The experiment involved 5 dietary levels of inulin (control: 0.2%, 0.4%, 0.8% inulin ). This experiment aimed at determining the effect of inulin on production perfermance. important bacterium in cecum and colon. pH in intestines and feces. apparent digestibility of calcium, activty of inulinase. and cholesterol in serum at the age of 21-49 days.1. The effect of inulin on pH and VFA in isolated culture trialThroughout the whole experiment, there was not significant increasing in pH from 0g/L to 2g/L, whereas significant increasing from 2g/L to 8g/L. Acetic acid showed a not significant difference among the 5 groups. However propionic acid among the inulin groups had significantly increased (P<0.05).2. The effect of inulin on production perfermence in piglets and broilersIn feeding trial of weaned piglets, the 1.5% inulin group had a significant (P<0.05) increasing average daily gain comparing with the 0.5% inulin group. Feed conversion rate of the 1% inulin group was significantly (P<0.05) lower than this of the 1.5% inulin group. Adding 0.5 inulin in diet, the diarrhea frequency of the weaned piglets was the lowest, the daily feed intake was the highest.In feeding trial of broilers, there was no significant difference in average daily gain and feed conversion rate at the age of 21-35 days among 4 groups. But at the age of 35-49 days, feed conversion rate in the 0.8% inulin group was reduced significantly (P<0.05) comparing with the other groups.3. The effect of inulin on VFA in cecum. pH in intestines and fecesIn feeding trial of weaned piglets, there was not significant difference in acetic acid and propionic acid. But compared to the control group. 1% inulin groups was significantly (P<0.05) low at propionic acid, significantly (P<0.05) high at Isobutyric acid and butyric acid. pH was reduced significantly (P<0.05) in cecum, colon, and rectum in the 1% inulin group. But in samll intestine, there was no significant difference in pH among 4 groups.In feeding trial of broilers, there was no significant difference in pH in samll intestine. Compared to the control group and 0.2% group, pH in cecum and feces at 0.4% and 08% group was significantly (P<0.0...
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