Research On Enzymatic-degradation Of Windbreak And Sand-Fixation Plant-Jerusalem Artichoke

Jerusalem artichoke belongs to helianthus genus. It has excellent ecological capacity of windbreak and sand-fixation because of its Wide adaptability to environment and good growth. Inulin rich in Jerusalem artichoke root is the potential carbohydrate source in fermentation industry. Inulin can be broke down into fructose or fructo-oligo-saccharides by inulinase and the product can be fermented into alcohol, organic acid. Insoluble chromogenic inulin gel was made through graft polymerization and staining with inulin, Glycidyl methacrylate(GMA) ,tetramethylethylenediamine(TMEDA)and active dye. 12 inulinase-producing microorganism were isolated and 1 highly inulinase-producing c strain was identified as Aspergillum niger . AU-55 with higher activity was obtained with complex mutagenesis. Optimized fermentation parameters through single and orthogonal experiments: Jerusalem artichoke juice 250.0 mL·L ^-1, corn steep liquor 20.0 g·L ^-1, NH4H2PO4 5.0 g·L ^-1,MgSO4·7H2O 0.5 g·L ^-1,NaCl 3.0 g·L ^-1, initial pH 6.5, inoculums 35 g·L ^-1, 30℃, 200r/min, fermentation time 4d and inulinase activity was up to 42.3 U·mL^-1. Optimized enzymatic degradation of Jerusalem artichoke juice were pH4.5, substrate content 10%, inulinase amount 12%, 45℃,14h and hydrolysis rate was up to 97%.