Study On Multiplication And Conservation Of Ginger In Vitro

Ginger is a vegetative plant. In recent years, the production of ginger becomes more and more important, when its planting area is extended with rising market. In this experiment, effective multiplication method, micro-ginger induction and germplasm conservation in vitro based on Shandong Laiwudajiang were studied. Tissue culture seedlings were got and favourable multiplication system was established. Through regulating sucrose, 6-BA, NAA, PP333 concentration in the medium and illumination condition, microrhizoms in vitro from ginger tissue culture seedlings were successfully induced.The main results are as followings:1. Effective multiplication method was set up. By adjusting the concentration of 6-BA and NAA in MS medium, MS+NAA0.1mg/l+6-BA3.5mg/L was effective for the shoot multiplication as a shoot inducing medium. In seedling subculture, MS+NAA0.1mg/l+6-BA2.0mg/L was available for the multiplication, and the rate is the highest. In bud subculture, the multiplication rate of MS+NAA0. 1 mg/l+6-BA1.5mg/L was the best one, and the rate is high. Bud subculture can produce new plantlets 4 times earlier than seedling culture.2. Induction of micro-ginger was researched. The inducing factors included the concentration of surcrose, 6-BA, NAA, PP333 and illumination condition. 6% surcrose concentration was best for the induction of micro-ginger. 6 -BA of 0.5mg/L, NAA of 0.1 mg/L, PP333 had promoting effect on microrhizom formation. 12h and 3500Lux illumination had a good effect on the induction. No illumination is a block for induction.3. The conservation of ginger plantlets based on micro-propagation was studied. The plantlets were cultured on 12 different media and conserved at 2500Lux(12h/d) and 25 + 1C for 5 months. The results showed that, the growth of the plantlets conserved on media with mannitol or sucrose could be restrained. The difference of sucrose concentration is prominence to the plantlets' height and the effect is better when the concentration is high, and the effect of mannitol was stronger than sucrose. So 30g/L sucrose and 20g/L mannitol is the best media for conserve ginger plantlets.