Rapid Propagation Of Stem Tip And Induction Of Micro-ginger In Kaempferia Glanga L.

The rhizome of Kaempferia glanga L.were used as materials to design method of single factor and orthogonal experiment,explored the effects of plant hormone ratio on stem tip growth,differentiation,subculture and rooting,the relationship between light intensity and stem tip proliferation.Moreover,we analyzed the effects of sucrose,hormone ratio and light on micro-ginger induction.A relatively complete technical system of tissue culture and micro-ginger induction was established to solve the problems of conventional asexual propagation.It solved the problems of conventional asexual reproduction,susceptibility to virus infection,resulting in decline in yield,quality and seed degeneration,and promotes the sustainable development of Kaempferia glanga L.industry.The main results were as follows:1.Sterile materials were obtained:0.1%HgCl₂ was sterilized for 10 minutes,the contamination rate was 28.32%,and the survival rate of stem tip was the highest （56.42%）,which was significantly higher than other treatments.2.Effects of 6-BA on explant germination in different parts（stem tip and axillary bud）.The results showed that,the redifferentiation coefficients of stem tip and axillary bud（3.21,2.56）were the highest,and the redifferentiation coefficients of stem tip was significantly higher than that of axillary bud in MS+6-BA 5 mg/L+NAA0.15 mg/L.3.The effects of KT、6-BA、6-BA and light intensity on stem tip growth and differentiation.The results showed that the redifferentiation coefficient of stem tip in MS+6-BA 5 mg/L+NAA 0.1 mg/L was the highest（2.54）which was significantly higher than other treatments.6-BA and light intensity treatments showed that the redifferentiation coefficients between 6-BA treatments and light treatments were significantly different.6-BA 4 mg/L and 2000 lx was the best for stem tips redifferentiation,MS+6-BA 4 mg/L+NAA 0.1 mg/L obtained the most differentiation buds in 2000 lx cultureand the redifferentiation coefficient was 7.77.4.The effects of 6-BA on adventitious bud proliferation were studied.Two adventitious buds（with and without leaf differentiation）differentiated from stem tip was proliferated.The results showed that the adventitious bud with leaf differentiation had the highest proliferation rate（7.29）in MS+NAA 0.05 mg/L+6-BA 4 mg/L,which was higher than that in 3 mg/L treatment;the stem tip without leaf differentiation had the highest proliferation rate（9.29）in MS+NAA 0.05 mg/L+6-BA 5 mg/L,which was significantly higher than that in 6-BA 1 mg/L treatment.5.The effect of NAA on adventitious bud rooting showed that MS+NAA 0.5 mg/L+6-BA 1 mg/L,rooting rate 100%,root number（19.82）,root length（1.55 cm）,root diameter（1.17 mm）were significantly higher than other treatments.6.The effects of sucrose on the induction of micro-ginger were studied.The results showed that（50-70）g/L sucrose,the largest number of micro-ginger （2.40³.02 g）,the heaviest（0.37⁰.47 g）,were significantly higher than those of 30g/L and 110 g/L treatments.7.The effects of of sucrose,6-BA,NAA and IAA on micro-ginger induction in vitro were studied,studied by L₉（3⁴）orthogonal table.The results showed that sucrose and NAA were the main factors affecting the formation of ginger in vitro.MS+sucrose60 g/L+6-BA 2 mg/L+NAA 0.8 mg/L+IAA1.1 mg/L was conducive to the formation of test-tube ginger,with the heaviest weight（0.68 g/bottle）and the largest quantity （3.58 bottles）.