| This paper introduced a new method for analyzing microsatellite DNA electrophoresis maps of polyploidy species using a computer-based program .Two identification models are used in this program, one is for single-lane and another for multi-lanes. With the single-lane model, integrated with the classification function, the program can analyze the target lanes, as well as the polyploid of each band based on the grayscale. With the multi-lane model, the program can analyze different lanes, and save the data of band position , band grayscale and molecular weight of each band, and ultimately build the (0,1) matrix which can be used for genetic distance analyses. The program can also be used to identify diploid DNA electrophoresis map.A case study of analyzing electrophoresis maps of Acipenser sinensis were implemented by the program. We used 365 groups of valid data as validation test, including 188 groups of haplploid band, 120 groups of diploid band, 32 groups of triploid band, and 25 groups of tetraploid band. Four groups of classification functions were computerd under the discrimination analysis. The correctly identified percentage of haplploid , diploid , triploid and tetraploid band discriminated by the classification functions was 85.1%, 90%, 28.1%, and 100% respectively.Of all bands combined the program identified 82.7% of the validation test set.The effectiveness of classification functions was examined by 120 bands as a test set. The correct percentage of haplploid , diploid , triploid and tetraploid band discriminated by the classification functions was 82.3%, 82.5%, 27.3%, and 100% respectively. Totally, the correct percentage discrimination by the function was 78.3%. Our findings demonstrated that the program is powerful to classify target lanes. With classification functions determined for other species which was inherited in tetrapolyploid mode., it can also be used to analyze their microsatellite DNA electrophoresis maps...
|
PDF Full Text Request