Studies On Establishment Of High Frequency Regeneration System And Transformation Of Salt-resistant Gene OsNHX1 Of Sophora

The regeneration systems for 8 Sophora genotypes were established. The regeneration ability and rooting rate of them were compared with different genotypes. The result showed that the proliferation rate of stem apex between genotypes differed little and the number of regenerated shoots was from 5 to 10; the ratio of buds regenerated from leaves varied much with a descending order var japonica Jinye(79.0%), var japonica golden (41.5%), Cladrastis sinensis (36.2%), Robinia psudoacian L.(34.2%), Cassia suffruticasa(25.8%), Sophora japonica L. (22.7%), var robinia tetrapkoid(21.3%) and Robinia hispida L.(18.9%), the descending order of rooting rate was var japonica Jinye(93.9%), var japonica golden(90.3%), Cladrastis sinensis (85.6%), Cassia suffruticasa(77.8%), Robinia psudoacian L.(76.5%), var robinia tetrapkoid (73.2%), Sophora japonica L.(70.4%) and Robinia hispida L.(40.0%). The regulatory effects and traits of genotypes on plant regeneration ability were discussed respectively by this experiment system. There were indications that the demand for basal medium and foreign hormone was identical between different genotypes of Sophora; the regulation of genotypes on calli induction and redifferentiation of Sophora explants was identical; the regulation of genotypes had identity on regeneration ability of plant tissues and organs, but there still existed specificities in different tissues and organs of different genotypes as well as the same genotype. The result not only put a foundation for proliferation of plantlets and gene transformation but also recovered the rules of regulation of genotypes on regeneration ability. Salt-resistant gene OsNHX1 was transformed into var Sophora Jinye by leaf disc with Agrobacterium tumefacien-mediated transformation. The regenerated shoots were acquired in selection culture media containing 20mg/LKanamycin. The resistant plantlets were tested by PCR analysis and were confirmed that the foreign DNA had been integrated into the genome of transgenic plants, which open the possibility to transfer salt-resistant genes into Sophora explants by Agrobacterium tumefacien-mediated transformation.