| Poplar is one of three fast-growing trees, it has an important position in the forestry industry in Hubei Province. Clones of'LH05-143','SN04-31'andLN05-51 are F1 hybrid generations, which grew well at the Institute of Experimental Forest of Populus in 'Shishou'City, Hubei Province. It also has significant heterosis, and has been testing of cultivars identification.In the experiment, the regeneration systems have established from petioles of 2 poplar clones(LH05-143 and SN04-31),and we also have study on transformation system of clone LN05-51,which transforms the insect-resistant gene Cry1C+9C by Agrobacterium. The main results that we have are summarized as follow:1.In the experiment of regeneration, the petioles of poplar were the best explants. The optimal sterilizing methods of petioles were dipping to 75% Ethanol for 30sec, and then were washed by steriled water 2 or 3 times, and then treating with 0.1% HgCl2 for 5min, the rate of contamination was 10%, and the rate of browning was 5% also.2.In the study of callus induction, the placement of the petioles had no significant effect on the rate of callus induction, but the number of induced callus were significantly different.and ultimately we choosed the style horizontally of the petioles of poplar clones as the best type in order to induce callus.3.In the indirect induction of regeneration, the optimal callus indcuction medium of LH05-143 and SN04-31 was MS+6-BA 0.2mg/L+NAA 1.0mg/L, and the callus were tight with green color. In another experiments, the optimal medium for the regenertion of LH05-143 from the callus was MS+6-BA 0.5mg/L+NAA 0.1mg/L, and SN04-31 was MS+6-BA 1.0mg/L+NAA 0.1mg/L.4. Optimal concentrations of 6-BA and NAA and their combinations were tested with quadratic regressive orthogonal design for their effect on the introduction for the adventitious shoots of Poplar clone LH05-143.We get a regression equation: y=2.5699+0.4101x1-0.2694x2+0.0093x1x2-0.9183x12-0.2537x22 Results showed that the best combination of the 6-BA and NAA was 1.75mg/L and 0.30mg/L. On the study of adventitious buds regeneration of SN04-31,the results showed that the optimal medium was MS+6-BA 0.5mg/L+NAA 0.1mg/L. 5.In the experiment of adventitious bud multiplication,the optimal medium for LH05-143 is MS+sucrose 30g/L+agar 6g/L+6-BA 0.1mg/L+NAA 0.05mg/L, and the optimal medium for SN04-31 is MS+sucrose 30g/L+Agar 7g/L+6-BA 0.1mg/L+ NAA 0.02mg/L.6.The optimal medium of rooting for LH05-143 is 1/2MS+IBA 0.5mg/L, and the mudium for SN04-31 is 1/2MS+IBA 0.2mg/L.7.Kanamycin sensitive experiments showed that the critical kanamycin sensitive concentrantions for inducing shoots and rooting of the poplar clone LN05-51 were 20mg/L and 15 mg/L.8.The best anti-bacterial methods for the transformation by Agrobacterium:selective medium with cefotaxime 400mg/L, and covered with a layer in the medium surface, and also need to chage the medium in time.9.By using Agrobacterium tumefaciens mediated to transformate the insect-resistant gene Cry1C+9C,there were no significant effects with acetosyringone.
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