Mechanism Of Quinoxaline-type Food Additive On Promoting-growth And Residue Of Kuianchun In Broiler Tissue

This article included 3 experiments which were used to study mechanism of quinoxaline-1,4-dioxide-type growth-promoting agents and kui`anchun and residue of kui`anchun in broiler tissue. Experiment1: effect of several quinoxaline-1,4-dioxide-type growth-promoting agents on performance of broiler and its mode of action .A feeding experiment was carried out over 42d with four groups of broiler chickens fed experimental diets formulated to provide no agent ,50 mg olaquindox ,50mg kui`anchun and 50mg kui xi tong .after 21d of the experiment, eight chickens from each pen were killed and the plasma total protein ,albumen ,RNA,RNA/DNA in muscle were measured .The conclusion suggested : (1)Average day gain ,average day food intake and food conversion in kui`anchun-fed were significantly higher than in olaquindox –fed and control (P<0.05); (2)No increase in plasma total protein and albumen were observe in control , kui`anchun-fed and kui xi tong-fed(P>0.05) ;however ,olaquindox –fed was significantly decrease(P<0.05); (3)significantly greater of RNA,RNA/DNA in muscle was demonstrated fed kui`anchun , kui xi tong, olaquindox than control(P<0.05) .Experiment2: effect of different level kui`anchun (0,50,75,100mg/kg ) on broiler and mechanism of growth-promoting .160 broiler 1day of age were randomly 4groups , which were fed 0,50,75,100mg/kg kui`anchun respectively .on 21d ,42d,56d of the experiment, eight chickens from each pen were killed and the plasma total protein ,albumen ,RNA,RNA/DNA in muscle ,long and weight of intestine were measured .Digestibility and metabolic experiment was carried out after 28d of feed experiment conclusion :(1) )Average day gain ,average day food intake and food conversion in 50mg/kg and 75mg/kg were significantly higher than in 100mg/kg and control(P<0.05);(2);The apparent digestibility of protein was higher in kui`anchun –fed (50mg/kg,75mg/kg)than control (P<0.05);(3) No increase in plasma total protein and albumen were observe in total groups(P>0.05) ;(4)weight of intestine was significantly smaller in 50mg/kg and 75mg/kg than in control(P<0.05) and100mg/kg,but not significant different about long of intestine (P>0.05);(5) significantly greater RNA,RNA/DNA in muscle was demonstrated fed kui`anchun than control(P<0.05) . Experiment3: broiler were fed kuianchun to measure the rudiment of kuianchun in liver ,kidney and muscle .The concentration of kuianchun was analyzed by liquid phase chromatography with RP-HPLC .The results showed that liver ,kidney and muscle of half life period of kuianchun were respectively 5.91,6.89 and 5.73 h chicken werefed it after sixtime .if residue concentration of kuianchun was 1ng/g which is safe for human body ,the phrase stopped kuianchun is 3day .