| In Ginkgo biloba L., Fozhi and Dongtinghuang, the two seed-purpose leadingcultivars, were used respectively as materials to randomly take seeds of 100 grains atinterval of 15d from the 20th day after full blossom for using RP-HPLC etc. to detectthe effective components contents such as carotenoid, flavonoid and Ginkgolides etc. inthe exotesta, and for study on effects of different methods of preservation andextraction of the exotesta to the effective components contents, and for comparing theseparated efficiencies of carotenoid and flavonoid in the exotesta by three pathways andmethods of HPTLC, PACCT and SGCCT, in order to further develop and utilize theexotesta resources. The above research results are described as follows:1.The content of carotenoid, flavonoid and Ginkgolides in the exotesta of both cultivarsof Fozhi and Dongtinghuang was 0.9863mg/g , 0.5238mg/g , 0.0595mg/g and1.2991mg, 0.5774mg/g, 0.0619mg/g respectively when Ginkgo's seed washarvested, in which the contents of different flavone glycosides were arranged asquercetin > keampferol > isorhamnetin, content of which is 0.1091mg/g ,0.0782mg/g, 0.0501mg/g and 0.1217mg/g, 0.1123mg/g, 0.0653mg/g respectively;the content of Ginkgolide B was more than that of Ginkgolide A, which was0.0828mg/g, 0.0372mg/g and 0.0875mg/g, 0.0363mg/g g respectively;2. Between Fozhi and Dongtinghuang, there were insignificant differences in contents of carotenoid, flavonoid and Ginkgolide B in the exotesta, but the significant differences in contents of three flavone glycosides such as quercetin, keampferol and isorhamnetin and Ginkgolide A appeared, the contents of three flavone glycosides and Ginkgolide A of the former was significantly higher than that of the latter;3. With growth of seed, the content of carotenoids in the exotesta was gradually increasing until the 125th day after full flower when it began increasing obviously from 0.5332mg/g at the 125th day to 1.1427mg/g at the 140th day; the content of flavonoid in the exotesta, the maximum of which appeared at the 65th day after fullblossom, then trended down, in which the changes of contents of quercetin, keampferol and isorhamnetin responded to that of flavonoid; the contents of Ginkgolide A and B, was lower at the starting, began increasing at the 35th day, speeded increasing from 0.02705mg/g of the 80th day to 0.06095mg/g of the 140th day after full blossom;4. The collected exotesta was treated by freeze drying, low temperature preservation and air drying, putting in room temperature respectively, the carotenoid content in the exotesta treated by the former was 4.62 times as many as that of the latter;5. The carotenoid content extracted by 4 solvents could be arranged as absolute ethyl ether > acetone > petroleum ether >the mixed solvent of 4 parts of petroleum ether plus 1 part of acetone; the results from the treatments with different extracting times indicated that the carotenoid content was increasing with increasing of extracting time which made the carotenoid contents extracted show as 180min>150min> 120min>90min>60min>30min, in the latter three treatments of which, there were not any significant differences, so based on the extractive efficiency, 120min was suitable to the time treatment for extracting the carotenoid from Ginkgo's exotesta under this experimental conditions-,6. In the treatments with different extracting methods, the content of flavonoid in the exotesta treated by backflow extracting and degreasing was remarkably more than that of extraction;7. In the different treatments of separating the flavone-like compounds, the separate efficiencies of both centrifugal thin-layer chromatography and polyamide column chromatography were better than that of silica gel chromatographic column;8. In the different treatments of separating the carotenoid, it was better for selecting the centrifugal thin-layer chromatography to separate the carotenoid because of loss of more carotenoid and time-consuming of column chromatography. Under this experimental conditions,...
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