Influence Of Cysteamine And Milk Replacer Proteins On The Development Of Small Intestinal Mucosa Of Goats

To gain insights into effects of cysteamine and nutrients on the development of small intestinal mucosa of goat, the following experiments were carried out:1) 8 male goats were utilized to illustrate the effects of orally administrated cysteamine compound (CT2000) on the growth of small intestinal mucosa. Goats were randomly divided into two groups: the control group (n=4) and experiment group (n=4). Goats from experiment group received CT2000 at a dosage of 45mg/Kg.BW.d. After a week adaptation and 4 weeks experiment, all goats were killed and sampled for morphology measurements. Results demonstrated that the villous height and crypts depth of duodenum and proximal jejunum were higher or tend to be higher'in experiment group than in control groups (P<0.05 or 0.05 0.10). The protein content and AP activity weretend to be higher in duodenum and proximal jejunum mucosa of experimental group; wihle the γ -GT activity and DNA content were significantly higher(P<0.05). Results indicated that orally administrated CT2000 to goats improves the mucosal morphology and accelerates the recovery from surgery.3) The dairy cow's intestinal epithelia cells in vitro were selected to evaluate the trophic actions of cysteamine. The cell proliferation and total anti-oxidation ability(T-AOC) and GSH content were investigated. Results showed that cysteamine with a dosage of 0.01mg/ml had strong stimulating effects on the proliferation; The T-AOC ability and GSH content were significantly higher in experimental group(P<0.05),suggested that cysteamine had a protective effect on intestinal epithelia cells in vitro.4) To determine the influences of different dietary protein sources on jejunal mucosa growth and development, seventeen neonatal goats at 7 d of age were used and assigned to two groups: casein group (8 lambs) and soya group (9 lambs). All lambs fed milk mixture containing either casein or soya protein during the experimental period. After 5 weeks, all the animals were slaughtered to obtain measurements of alkaline phosphatase (AP) activity, protein and nucleic acid content of jejunal mucosa. The protein content and AP activity were significantly higher (P<0.05) or tend to be higher (P<0.10) for casein group in all three sections: the proximal (JPM), media (JMM) and distal (JDM) jejunal mucosa. And there's a significant proximodistal gradient for all the two groups. JPM had a higher protein content and AP activity than both JMM and JDM (P<0.01). The content of RNA and DNA were not significantly affected by dietary protein sources with the exception of JPM, in which casein group had a higher RNA content than soya group. While c...